High-throughput screening by using a blue-fluorescent antibody sensor

Blue lights the way! The rapid evaluation of enantiomeric excess in the high-throughput screening of libraries has been a hurdle to the discovery of effective catalysts. A blue-fluorescent monoclonal antibody (mAb) addresses this problem; mAb 19G2 is used as a fluorescent sensor (see picture) to evaluate a panel of Cinchona alkaloid derivatives in the synthesis of asymmetric amino acids by phase-transfer catalysis.