Isolation and characterization of phosphate solubilizing bacteria from the rhizosphere of crop plants of Korea

被引:232
作者
Chung, H
Park, M
Madhaiyan, M
Seshadri, S
Song, J
Cho, H
Sa, T
机构
[1] Chungbuk Natl Univ, Dept Agr Chem, Cheongju 361763, Chungbuk, South Korea
[2] Natl Inst Agr Biotechnol, Korean Agr Culture Collect, Suwon 441707, South Korea
关键词
phosphate solubilization; Pantoea agglomerans; Enterobacter aerogenes; Klebsiella sp;
D O I
10.1016/j.soilbio.2005.02.025
中图分类号
S15 [土壤学];
学科分类号
0903 ; 090301 ;
摘要
Whole-cell fatty acids methyl ester (FAME) profile and 16S rDNA sequence analysis were employed to isolate and identify the bacterial groups that actively solubilized phosphates in vitro from rhizosphere soil of various crops of Korea. Out of several hundred colonies that grew on Pikovskaya's medium 13 best isolates were selected based on the solubilization of insoluble phosphates in liquid culture and further characterized and identified. They were clustered under the genera Enterobacter, Pantoea and Klebsiella and the sequences of three representative strains were deposited in the GenBank nucleotide sequence data library under the accession numbers AY335552, AY335553, AY335554.(c) 2005 Published by Elsevier Ltd.
引用
收藏
页码:1970 / 1974
页数:5
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