In situ monitoring of chitin degradation by Streptomyces lividans pCHIO12 within Enchytraeus crypticus (Oligochaeta) feeding on Aspergillus proliferans

被引：5

作者：

Kristufek, V

Fischer, S

Bührmann, J

Zeltins, A

Schrempf, H

机构：

[1] Univ Osnabruck, Fachbereich Biol Chem, D-49069 Osnabruck, Germany

[2] Acad Sci Czech Republ, Inst Soil Biol, CR-37005 Ceske Budejovice, Czech Republic

来源：

FEMS MICROBIOLOGY ECOLOGY | 1999年 / 28卷 / 01期

关键词：

chitinase; chitin-binding protein; streptomycetes; ascomycetes;

D O I：

10.1016/S0168-6496(98)00090-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Enchytraeus crypticus (Oligochaeta) subsists on the fungus Aspergillus proliferans. The proliferation can be considerably enhanced by co-feeding the Gram-positive bacterium Streptomyces lividans pCHIO12 overproducing an exochitinase. In situ hybridizations with 23S rRNA-targeting, fluorescein-labelled oligonucleotides revealed that S. lividans pCHIO12 survives well within the gut of E. crypticus. Immunological analyses using a rhodamine-labelled alpha-chitin-binding protein (CHB1) allowed the conclusion that the bacterial enzyme degrades chitin within hyphae of A. proliferans. Our data show that the established in situ monitoring methods are valuable tools to analyze interactions between Enchytraeidae and microorganisms. (C) 1999 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.

引用

页码：41 / 48

页数：8

共 38 条

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