A newly identified extrinsic input triggers a distinct gastric mill rhythm via activation of modulatory projection neurons

被引:50
作者
Blitz, Dawn M. [1 ]
White, Rachel S. [1 ]
Saideman, Shari R. [1 ]
Cook, Aaron [1 ]
Christie, Andrew E. [2 ,3 ]
Nadim, Farzan [4 ,5 ]
Nusbaum, Michael P. [1 ]
机构
[1] Univ Penn, Dept Neurosci, Sch Med, Philadelphia, PA 19104 USA
[2] Univ Washington, Dept Biol, Seattle, WA 98195 USA
[3] Mt Desert Isl Biol Lab, Salsbury Cove, ME 04672 USA
[4] New Jersey Inst Technol, Dept Math Sci, Newark, NJ 07102 USA
[5] Rutgers State Univ, Dept Biol Sci, Newark, NJ 07102 USA
关键词
neuromodulation; central pattern generator; projection neurons; neuropeptide; Cancer borealis;
D O I
10.1242/jeb.015222
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
Neuronal network flexibility enables animals to respond appropriately to changes in their internal and external states. We are using the isolated crab stomatogastric nervous system to determine how extrinsic inputs contribute to network flexibility. The stomatogastric system includes the well-characterized gastric mill ( chewing) and pyloric ( filtering of chewed food) motor circuits in the stomatogastric ganglion. Projection neurons with somata in the commissural ganglia (CoGs) regulate these rhythms. Previous work characterized a unique gastric mill rhythm that occurred spontaneously in some preparations, but whose origin remained undetermined. This rhythm includes a distinct protractor phase activity pattern, during which a key gastric mill circuit neuron (LG neuron) and the projection neurons MCN1 and CPN2 fire in a pyloric rhythm-timed activity pattern instead of the tonic firing pattern exhibited by these neurons during previously studied gastric mill rhythms. Here we identify a new extrinsic input, the post-oesophageal commissure (POC) neurons, relatively brief stimulation (30 s) of which triggers a long-lasting (tens of minutes) activation of this novel gastric mill rhythm at least in part via its lasting activation of MCN1 and CPN2. Immunocytochemical and electrophysiological data suggest that the POC neurons excite MCN1 and CPN2 by release of the neuropeptide Cancer borealis tachykinin-related peptide la (CabTRP la). These data further suggest that the CoG arborization of the POC neurons comprises the previously identified anterior commissural organ (ACO), a CabTRP la-containing neurohemal organ. This endocrine organ thus appears to also have paracrine actions, including activation of a novel and lasting gastric mill rhythm.
引用
收藏
页码:1000 / 1011
页数:12
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