Thiazole orange as the fluorescent intercalator in a high resolution FID assay for determining DNA binding affinity and sequence selectivity of small molecules

被引：91

作者：

Boger, DL

Tse, WC

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Skaggs Inst Chem Biol, La Jolla, CA 92037 USA

来源：

BIOORGANIC & MEDICINAL CHEMISTRY | 2001年 / 9卷 / 09期

关键词：

D O I：

10.1016/S0968-0896(01)00243-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The viability of using thiazole orange as an alternative to ethidium bromide in a fluorescent intercalator displacement (FID) assay is explored by profiling the DNA binding affinity and sequence selectivity of netropsin. Utilizing a library of hairpin deoxyoligonucleotides containing all possible four base-pair sequences, the method provides a high resolution profile of the DNA binding properties of small molecules in a high throughput format. (C) 2001 Elsevier Science Ltd. All rights reserved.

引用

收藏

页码：2511 / 2518

页数：8

相关论文

共 67 条

[1] DNA-SEQUENCE PREFERENCES OF SEVERAL AT-SELECTIVE MINOR-GROOVE BINDING LIGANDS [J].

ABUDAYA, A ;

BROWN, PM ;

FOX, KR .

NUCLEIC ACIDS RESEARCH, 1995, 23 (17) :3385-3392

[2] HETERONUCLEAR 2-DIMENSIONAL N-15-NMR AND C-13-NMR STUDIES OF DNA OLIGOMERS AND THEIR NETROPSIN COMPLEXES USING INDIRECT PROTON DETECTION [J].

ASHCROFT, J ;

LIVE, DH ;

PATEL, DJ ;

COWBURN, D .

BIOPOLYMERS, 1991, 31 (01) :45-55

[3] Sequence-specific DNA minor groove binders. Design and synthesis of netropsin and distamycin analogues [J].

Bailly, C ;

Chaires, JB .

BIOCONJUGATE CHEMISTRY, 1998, 9 (05) :513-538

[4] Total synthesis of distamycin A and 2640 analogues: A solution-phase combinatorial approach to the discovery of new, bioactive DNA binding agents and development of a rapid, high-throughput screen for determining relative DNA binding affinity or DNA binding sequence selectivity [J].

Boger, DL ;

Fink, BE ;

Hedrick, MP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2000, 122 (27) :6382-6394

[5] A simple, high-resolution method for establishing DNA binding affinity and sequence selectivity [J].

Boger, DL ;

Fink, BE ;

Brunette, SR ;

Tse, WC ;

Hedrick, MP .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2001, 123 (25) :5878-5891

[6] Assessment of solution-phase positional scanning libraries based on distamycin A for the discovery of new DNA binding agents [J].

Boger, DL ;

Dechantsreiter, MA ;

Ishii, T ;

Fink, BE ;

Hedrick, MP .

BIOORGANIC & MEDICINAL CHEMISTRY, 2000, 8 (08) :2049-2057

[7]

BRESLAUER KJ, 1988, STRUCTURE EXPRESSION, V2, P273

[8] MICROGONOTROPENS AND THEIR INTERACTIONS WITH DNA .2. QUANTITATIVE-EVALUATION OF EQUILIBRIUM-CONSTANTS FOR 1/1 AND 2/1 BINDING OF DIEN-MICROGONOTROPEN-A, DIEN-MICROGONTROPEN-B, AND DIEN-MICROGONTROPEN-C AS WELL AS DISTAMYCIN AND HOECHST-33258 TO D(GGCGCAAATTTGGCGG)/D(CCGCCAATTTGCGCC) [J].

BROWNE, KA ;

HE, GX ;

BRUICE, TC .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1993, 115 (16) :7072-7079

[9]

BROWNE MJ, 1986, GENOMES MOL BIOL DRU

[10] A comparison of DNA-binding drugs as inhibitors of E2F1- and Sp1-DNA complexes and associated gene expression [J].

Chiang, SY ;

Azizkhan, JC ;

Beerman, TA .

BIOCHEMISTRY, 1998, 37 (09) :3109-3115

← 1 2 3 4 5 6 7 →