Chiral stationary phase based on a biostable L-RNA aptamer

被引：53

作者：

Brumbt, A ^{[1
]}

Ravelet, C ^{[1
]}

Grosset, C ^{[1
]}

Ravel, A ^{[1
]}

Villet, A ^{[1
]}

Peyrin, E ^{[1
]}

机构：

[1] Univ Grenoble 1, Inst Chim Mol Grenoble, CNRS, Dept Pharmacochim Mol,UMR 5063,FR 2607,UFR Pharm, F-38240 Meylan, France

来源：

ANALYTICAL CHEMISTRY | 2005年 / 77卷 / 07期

关键词：

D O I：

10.1021/ac048344l

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An immobilized anti-L-arginine D-RNA aptamer, used as a target-specific chiral stationary phase (CSP), was found to be very quickly degraded by RNases under usual chromatographic utilization and storage. To overcome this severe limitation for a practical use, a CSP based on the L-RNA aptamer, that is, the mirror image of the D-RNA aptamer, was created. It was shown that this mirror-image approach was a very simple and powerful strategy to develop a highly stable stationary phase due to the intrinsic insensitivity of L-RNA to the RNase degradation. In addition, such an approach allowed one to reverse the enantiomer elution order relative to that obtained with the corresponding D-RNA CSP.

引用

页码：1993 / 1998

页数：6

共 36 条

[1] Structural probing and damage selection of citrulline- and arginine-specific RNA aptamers identify base positions required for binding [J].