Poly(ADP-ribose) polymerase-1 is required for efficient HIV-1 integration

被引：85

作者：

Ha, HC

Juluri, K

Zhou, Y

Leung, S

Hermankova, M

Snyder, SH ^{[1
]}

机构：

[1] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA

[2] Johns Hopkins Univ, Sch Med, Dept Med, Baltimore, MD 21205 USA

[3] Johns Hopkins Univ, Sch Med, Dept Pharmacol & Mol Sci, Baltimore, MD 21205 USA

[4] Johns Hopkins Univ, Sch Med, Dept Psychiat & Behav Sci, Baltimore, MD 21205 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2001年 / 98卷 / 06期

关键词：

D O I：

10.1073/pnas.051633498

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Poly(ADP-ribose) polymerase-1 (PARP-1; EC 2.4.2.30) is an abundant nuclear enzyme, activated by DNA strand breaks to attach up to 200 ADP-ribose groups to nuclear proteins. As retroviral infection requires integrase-catalyzed DNA strand breaks, we examined infection of pseudotyped HIV type I in fibroblasts from mice with a targeted deletion of PARP-1. Viral infection is almost totally abolished in PARP-1 knockout fibroblasts. This protection from infection reflects prevention of viral integration into the host genome. These findings suggest a potential for PARP inhibitors in therapy of HIV type I infection.

引用

页码：3364 / 3368

页数：5

共 53 条

[1] Identification of poly(ADP-ribose) polymerase as a transcriptional coactivator of the human T-cell leukemia virus type 1 tax protein
Anderson, MG
Scoggin, KES
Simbulan-Rosenthal, CM
Steadman, JA
[J]. JOURNAL OF VIROLOGY, 2000, 74 (05) : 2169 - 2177
[2] DNA-dependent protein kinase is not required for efficient lentivirus integration
Baekelandt, V
Claeys, A
Cherepanov, P
De Clercq, E
De Strooper, B
Nuttin, B
Debyser, Z
[J]. JOURNAL OF VIROLOGY, 2000, 74 (23) : 11278 - 11285
[3] Production of high-titer human immunodeficiency virus type 1 pseudotyped with vesicular stomatitis virus glycoprotein
Bartz, SR
Vodicka, MA
[J]. METHODS-A COMPANION TO METHODS IN ENZYMOLOGY, 1997, 12 (04): : 337 - 342
[4] Antisense oligonucleotides: Is the glass half full or half empty?
Bennett, CF
[J]. BIOCHEMICAL PHARMACOLOGY, 1998, 55 (01) : 9 - 19
[5] SYMPOSIUM - CELLULAR-RESPONSE TO DNA DAMAGE - THE ROLE OF POLY(ADP-RIBOSE) - POLY(ADP-RIBOSE) IN THE CELLULAR-RESPONSE TO DNA DAMAGE
BERGER, NA
[J]. RADIATION RESEARCH, 1985, 101 (01) : 4 - 15
[6] Use of a repetitive mouse B2 element to identify transplanted mouse cells in mouse-chick chimeras
Bollag, RJ
Crawford, KB
Stadt, H
Kumiski, D
Zdanowicz, M
Baptista, C
Herlea, V
Kirby, ML
[J]. EXPERIMENTAL CELL RESEARCH, 1999, 248 (01) : 75 - 78
[7] Mice lacking the poly(ADP-ribose) polymerase gene are resistant to pancreatic beta-cell destruction and diabetes development induced by streptozocin
Burkart, V
Wang, ZQ
Radons, J
Heller, B
Herceg, Z
Stingl, L
Wagner, EF
Kolb, H
[J]. NATURE MEDICINE, 1999, 5 (03) : 314 - 319
[8] DNA STRAND BREAKS, NAD METABOLISM, AND PROGRAMMED CELL-DEATH
CARSON, DA
SETO, S
WASSON, DB
CARRERA, CJ
[J]. EXPERIMENTAL CELL RESEARCH, 1986, 164 (02) : 273 - 281
[9] Presence of an inducible HIV-1 latent reservoir during highly active antiretroviral therapy
Chun, TW
Stuyver, L
Mizell, SB
Ehler, LA
Mican, JAM
Baseler, M
Lloyd, AL
Nowak, MA
Fauci, AS
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1997, 94 (24) : 13193 - 13197
[10] INHIBITION OF HIV-1-IIIB REPLICATION IN AA-2 AND MT-2 CELLS IN CULTURE BY 2 LIGANDS OF POLY (ADP-RIBOSE) POLYMERASE - 6-AMINO-1,2-BENZOPYRONE AND 5-IODO-6-AMINO-1,2-BENZOPYRONE
COLE, GA
BAUER, G
KIRSTEN, E
MENDELEYEV, J
BAUER, PI
BUKI, KG
HAKAM, A
KUN, E
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1991, 180 (02) : 504 - 514

← 1 2 3 4 5 6 →