Cloning and expression of human lectin-like oxidized low density lipoprotein receptor-1 in Pichia pastoris

被引：5

作者：

Huang, ZT

Zhang, TT

Yang, JL

Zhu, P ^{[1
]}

Du, GH

Cheng, KD

机构：

[1] Chinese Acad Med Sci, Inst Mat Med, Dept Biosynth Nat Prod, Beijing 100050, Peoples R China

[2] Chinese Acad Med Sci, Inst Mat Med, Natl Pharmaceut Screening Ctr, Beijing 100050, Peoples R China

来源：

BIOTECHNOLOGY LETTERS | 2005年 / 27卷 / 01期

基金：

中国国家自然科学基金;

关键词：

heterologous expression; LOX-1; Pichia pastoris; recombinant protein;

D O I：

10.1007/s10529-004-6582-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Lectin-like oxidatively-modified LDL receptor-1 (LOX-1) is a major receptor for oxidized low-density lipoprotein (oxLDL) in aortic endothelial cells. Human LOX-1 (hLOX-1) gene (cDNA) was cloned from the monocytic leukemic cell line THP-1 and expressed in Pichia pastoris. The recombinant protein (rhLOX-1) was purified by his-tag affinity chromatography. Preliminary identification was performed by Western blot analysis and a ligand-receptor binding assay showed that the protein had specific oxLDL-binding activity.

引用

页码：49 / 52

页数：4

共 8 条

[1] Increased expression of lectinlike oxidized low density lipoprotein receptor-1 in initial atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits [J].