Detection of foodborne pathogens using surface plasmon resonance biosensors

被引：194

作者：

Koubová, V

Brynda, E

Karasová, L

Skvor, J

Homola, J

Dostálek, J

Tobiska, P

Rosicky, J

机构：

[1] Acad Sci Czech Republ, Inst Macromol Chem, CR-16206 Prague, Czech Republic

[2] Inst Chem Technol, CR-16628 Prague, Czech Republic

[3] SEVA IMUNO Praha, Prague 16100, Czech Republic

[4] Acad Sci Czech Republ, Inst Radio Engn & Elect, CR-18251 Prague, Czech Republic

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 2001年 / 74卷 / 1-3期

关键词：

foodborne pathogens; surface plasmon resonance; immunosensors;

D O I：

10.1016/S0925-4005(00)00717-6

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

Salmonella enteritidis and Listeria monocytogenes were detected in real time using a surface plasmon resonance (SPR) sensor based on prism excitation of surface plasmons and spectral interrogation. The respective antibodies against the pathogens were immobilized on the gold sensor surface as a covalently crosslinked double-layer or covalently bound on a cross-linked albumin layer. Salmonella and Listeria could be detected by the sensor at concentrations down to 10(6) cell/ml. The sensor sensitivity was comparable with that of standard ELISA in which the same antibodies were used. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：100 / 105

页数：6

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