Dimethylsulfoxide-stabilized conformer of guanine-adenine repeat strand of DNA

被引：11

作者：

Kypr, J ^{[1
]}

Vorlícková, M ^{[1
]}

机构：

[1] Acad Sci Czech Republ, Inst Biophys, CZ-61265 Brno, Czech Republic

来源：

BIOPOLYMERS | 2001年 / 62卷 / 02期

关键词：

DNA; conformation; dimethylsulfoxide; alpha helix; dimerization;

D O I：

10.1002/bip.1000

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Circular dichroism spectroscopy and other methods are used to show that the addition of dimethylsulfoxide causes reversible folding of the (GA)(10) strand of DNA into an ordered single-stranded conformer. The ordered conformer melts in a cooperative way and it does not contain protonated adenine. The (TA)(10), (A)(20), and (G)(20) are all unstable in this conformer. To the best of our knowledge, this is the first known ordered conformer of DNA that is stabilized by dimethylsulfoxide. This conformer might be a DNA analog of the protein alpha helix, which is an interesting idea for thinking about the evolution of DNA. (C) 2001 John Wiley & Sons, Inc.

引用

页码：81 / 84

页数：4

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