Clinical evaluation of the Amplified Mycobacterium Tuberculosis Direct 2 test (AMTD-2, GenProbe) for rapid diagnosis of tuberculosis

被引:3
作者
Artiles, F [1 ]
Pena, MJ [1 ]
Campos-Herrero, MI [1 ]
Lafarga, B [1 ]
机构
[1] Hosp Gran Canaria Dr Negrin, Microbiol Serv, Las Palmas Gran Canaria 35020, Spain
来源
ENFERMEDADES INFECCIOSAS Y MICROBIOLOGIA CLINICA | 2001年 / 19卷 / 02期
关键词
Mycobacterium tuberculosis AMTD-2 respiratory samples; nonrespiratory samples;
D O I
10.1016/S0213-005X(01)72560-8
中图分类号
R51 [传染病];
学科分类号
100401 ;
摘要
OBJETIVE. To evaluate the performance of the Amplified Mycobacterium tuberculosis Direct Test 2- Gen Probe (AMTD- 2) for direct detection of Mycobacterium tuberculosis in smear-negative samples. PATIENTS AND METHODS. From January to December 1999. 683 specimens, 333 respiratory and 350 non-respiratory ones collected from 457 patients, were included in the study. All the samples of HIV-positive patients, the respiratory samples from patients suspected of having pulmonary tuberculosis (at least two by patient) and all non-respiratory samples were included. As diagnosis method of reference, the culture isolation was considered. Clinical data were analyzed in case of discrepant results and clinical diagnosis was considered the reference criteria. The technique was performed once a week. RESULTS. The sensitivity, specificity, and positive and negative predictive values of this assay were 58.9%, 93.9%, 37.1% and 97.4% respectively related to the standard culture. When referred to clinical diagnosis of active tuberculosis, these values improved to 70.4%, 97.7%, 73.1% and 96.8% respectively (in respiratory samples were 67.6%, 98.6%, 86.2% and 95.9% and in nonrespiratory ones 76.5%, 96.9%, 56.5% and 98.7% respectively). The mean time of diagnosis by culture and by AMTD-2 were 20.3 days (range 10-63) and 5.7 days (range 2-20) respectively. DISCUSSION. It is concluded that AMTD-2 is a rapid diagnosis method when clinical data are sugestive with active tuberculosis. However, due to the low positive predictive value, it would be convenient to obtain successive samples to confirm the result in patients without clinical evidence of tuberculosis.
引用
收藏
页码:53 / 56
页数:4
相关论文
共 22 条
[1]   DIAGNOSTIC-CRITERIA FOR TUBERCULOUS MENINGITIS AND THEIR VALIDATION [J].
AHUJA, GK ;
MOHAN, KK ;
PRASAD, K ;
BEHARI, M .
TUBERCLE AND LUNG DISEASE, 1994, 75 (02) :149-152
[2]  
Alonso P, 1998, EUR J CLIN MICROBIOL, V17, P371
[3]   SCREENING OF RESPIRATORY-TRACT SPECIMENS FOR THE PRESENCE OF MYCOBACTERIUM-TUBERCULOSIS BY USING THE GEN-PROBE AMPLIFIED MYCOBACTERIUM-TUBERCULOSIS DIRECT TEST [J].
BODMER, T ;
GURTNER, A ;
SCHOPFER, K ;
MATTER, L .
JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (06) :1483-1487
[4]   Clinical efficacy of the amplified Mycobacterium tuberculosis direct test for the diagnosis of pulmonary tuberculosis [J].
Bradley, SP ;
Reed, SL ;
Catanzaro, A .
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE, 1996, 153 (05) :1606-1610
[5]   Evaluation of three commercial detection systems for Mycobacterium tuberculosis where clinical diagnosis is difficult [J].
Brown, TJ ;
Power, EGM ;
French, GL .
JOURNAL OF CLINICAL PATHOLOGY, 1999, 52 (03) :193-197
[6]  
Catanzaro A, 1997, AM J RESP CRIT CARE, V155, P1804, DOI 10.1164/ajrccm.155.5.9154896
[7]  
Chapin Kimberle, 1995, P33
[8]   Routine use of the gen-probe MTD2 amplification test for detection of Mycobacterium tuberculosis in clinical specimens in a large public health mycobacteriology laboratory [J].
Chedore, P ;
Jamieson, FB .
DIAGNOSTIC MICROBIOLOGY AND INFECTIOUS DISEASE, 1999, 35 (03) :185-191
[9]   Diagnosis of extrapulmonary tuberculosis by Gen-Probe amplified Mycobacterium tuberculosis direct test [J].
Ehlers, S ;
Ignatius, R ;
Regnath, T ;
Hahn, H .
JOURNAL OF CLINICAL MICROBIOLOGY, 1996, 34 (09) :2275-2279
[10]  
Fairfax MR, 1996, AM J CLIN PATHOL, V106, P594