Ionic polysaccharide hydrogels via the passerini and ugi multicomponent condensations: Synthesis, behavior and solid-state NMR characterization

被引:70
作者
de Nooy, AEJ
Capitani, D
Masci, G
Crescenzi, V
机构
[1] Univ Roma La Sapienza, Dept Chem, I-00185 Rome, Italy
[2] CNR, Inst Nucl Chem, Res Area Rome, I-00016 Monterotondo, Rome, Italy
[3] CNR, NMR Serv, Res Area Rome, I-00016 Monterotondo, Rome, Italy
关键词
D O I
10.1021/bm005517h
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Original data are provided demonstrating that the title condensations are simple and versatile methods for the synthesis of hydrogels based on a variety of carboxylated polysaccharides. In this work, the biopolymers considered are sodium hyaluronate and sodium alginate. Nonnatural carboxylated polysaccharides were commercial (carboxymethyl)cellulose or were obtained by carboxymethylation or selective oxidation of primary alcohol groups of scleroglucan and dextran. Hydrogels prepared via the Passerini reaction were transparent, alkali labile materials whereas the transparency of the Ugi gels depended on the polysaccharide, the cross-linker, and the degree of cross-linking. The Ugi gels were stable for several months at a pH ranging from 1.3 to 11 and up to temperatures over 90 degreesC. The structure of the networks was studied by means of C-13 CP-MAS and N-15 CP-MAS NMR spectroscopy. A quantitative NMR analysis and elemental analysis of the dry gels allowed us to estimate the efficiency of the reactions, i.e., the actual degree of cross-linking, which appeared to be about 80% of theoretical. The influence of added salt and pH on the swelling of several Ugi gels with different degrees of cross-linking was studied in a qualitative manner.
引用
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页码:259 / 267
页数:9
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