Pseudomonas aeruginosa lectins I and II and their interaction with human airway cilia

被引:42
作者
Mewe, M
Tielker, D
Schönberg, R
Schachner, M
Jaeger, KE
Schumacher, U
机构
[1] Univ Hamburg, Klinikum Eppendorf, Inst Anat Expt Morphol 2, D-20246 Hamburg, Germany
[2] Univ Hamburg, Klinikum Eppendorf, ENT Klin, D-20246 Hamburg, Germany
[3] Univ Dusseldorf, Inst Mol Enzymtechnol, D-4000 Dusseldorf, Germany
[4] Univ Hamburg, Zentrum Mol Neurobiol, Hamburg, Germany
关键词
bacterial adhesion; cilia; cystic fibrosis; lectins; Pseudomonas aeruginosa; respiratory tract infections;
D O I
10.1258/0022215054516313
中图分类号
R76 [耳鼻咽喉科学];
学科分类号
100213 ;
摘要
The bacterium Pseudomonas aeruginosa (PA) produces two carbohydrate binding lectins, designated PA lectin-I and lectin-II (PA-IL, PA-IIL). Both lectins are used by the bacterium to adhere to the glycocalyx of mammalian cells. In addition, the lectins immobilize ciliary beat. The kinetics of ciliary beat inhibition by each individual lectin have been analysed; however, their joint action on cilia has not been reported. Here we demonstrate that PA-IL and PA-IIL inhibit ciliary beat in a similar time-dependent manner. If applied simultaneously, ciliary beat inhibition after five hours of incubation was weaker than if each lectin was applied separately. Thus it can be hypothesized that the lectins compete for the same binding site(s) of the glycocalyx. Sugar inhibition experiments demonstrate that D-galactose and L-fucose inhibit both lectins, although clear preferences of D-galactose for PA-IL and of L-fucose for PA-IIL exist. These interactions have to be kept in mind when designing sugar-based therapies.
引用
收藏
页码:595 / 599
页数:5
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