Folic acid-mediated targeting of cowpea mosaic virus particles to tumor cells

被引：189

作者：

Destito, Giuseppe

Yeh, Robert

Rae, Chris S.

Finn, M. G.

Manchester, Marianne ^{[1
]}

机构：

[1] Scripps Res Inst, Dept Chem, La Jolla, CA 92037 USA

[2] Scripps Res Inst, Dept Cell Biol, La Jolla, CA 92037 USA

[3] Scripps Res Inst, Dept Ctr Integrat Mol Biosci, La Jolla, CA 92037 USA

[4] Univ Magna Gracia Catanzaro, Dipartimento Med Sperimentale & Clin, I-88100 Catanzaro, Italy

来源：

CHEMISTRY & BIOLOGY | 2007年 / 14卷 / 10期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/j.chembiol.2007.08.015

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cowpea mosaic virus (CPMV) is a well-characterized nanoparticle that has been used for a variety of nanobiotechnology applications. CPMV interacts with several mammalian cell lines and tissues in vivo. To overcome natural CPMV targeting and redirect CPMV particles to cells of interest, we attached a folic acid-PEG conjugate by using the copper-catalyzed azide-alkyne cycloaddition reaction. PEGylation of CPMV completely eliminated background binding of the virus to tumor cells. The PEG-folate moiety allowed CPMV-specific recognition of tumor cells bearing the folate receptor. In addition, by testing CPMV formulations with different amounts of the PEG-FA moiety displayed on the surface, we show that higher-density loading of targeting ligands on CPMV may not be necessary for efficient targeting to tumor cells. These studies help to define the requirements for efficiently targeting nanoparticles and protein cages to tumors.

引用

页码：1152 / 1162

页数：11

共 87 条

[1] Persubstituted cyclodextrin-based glycoclusters as inhibitors of protein-carbohydrate recognition using purified plant and mammalian lectins and wild-type and lectin-gene-transfected tumor cells as targets [J].