Laparoscopic surgery and Kupffer cell activation

被引:19
作者
Vittimberga, FJ [1 ]
Nolan, B [1 ]
Perugini, RA [1 ]
Spector, L [1 ]
Callery, MP [1 ]
机构
[1] Univ Massachusetts, Sch Med, Dept Surg, Worcester, MA 01655 USA
来源
SURGICAL ENDOSCOPY-ULTRASOUND AND INTERVENTIONAL TECHNIQUES | 2000年 / 14卷 / 12期
关键词
interleukin-6; laparoscopy; macrophages; signal transduction; TNF-alpha;
D O I
10.1007/s004640010065
中图分类号
R61 [外科手术学];
学科分类号
摘要
Background: Evidence tends to support a relative preservation of the systemic immune response with laparoscopy as compared with laparotomy. However, the role of hepatic macrophages, or Kupffer cells, in modulating this immune advantage is unknown. This study investigated the functions of Kupffer cells after either laparoscopy or laparotomy in a rat model. Methods: Rats underwent laparoscopy, laparotomy, or control operations. Kupffer cells were harvested, cultured, and stimulated with lipopolysaccharide. Culture supernatants were analyzed for tumor necrosis factor (TNF-alpha) and interleukin-6 (IL-6). Cytoplasmic lysates were analyzed for activation of two mitogen-activated protein kinases (MAPKs). Results: Production of TNF-alpha and IL-6 was similar in laparoscopy, laparotomy, and control groups. Both laparotomy and laparoscopy showed increased activation of p38 MAPK as compared with controls. Activation of ERK1/2 was decreased during laparotomy as compared with laparoscopy. Conclusions: Although cytokine production was similar in the laparoscopy and laparotomy groups, changes in MAPK activation suggest that intracellular pathways are more affected during laparotomy than during laparoscopy.
引用
收藏
页码:1171 / 1176
页数:6
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