Detection of Salmonella enteritidis in shell and liquid eggs using enrichment and plating

被引：21

作者：

Hara-Kudo, Y

Kumagai, S

Masuda, T

Goto, K

Ohtsuka, K

Masaki, H

Tanaka, H

Tanno, K

Miyahara, M

Konuma, H

机构：

[1] Natl Inst Hlth Sci, Div Microbiol, Setagaya Ku, Tokyo 1588501, Japan

[2] Natl Inst Infect Dis, Dept Biomed Food Res, Shinjuku Ku, Tokyo 1628640, Japan

[3] Shizuoka Inst Environm & Hyg, Shizuoka 4208637, Japan

[4] Niigata Prefectural Res Lab Hlth & Environm, Niigata 950215, Japan

[5] Saitama Inst Publ Hlth, Urawa, Saitama 338, Japan

[6] Japan Food Res Labs, Shibuya Ku, Tokyo 1518501, Japan

来源：

INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY | 2001年 / 64卷 / 03期

关键词：

Salmonella enteritidis; egg; detection; enrichment; plating;

D O I：

10.1016/S0168-1605(00)00475-X

中图分类号：

TS2 [食品工业];

学科分类号：

0832 ;

摘要：

Detection methods using various enrichment and plating media and immunoconcentration for Salmonella enteritidis in shell and liquid eggs were evaluated. For liquid egg samples naturally contaminated with S. enteritidis, pre-enrichment in 225 ml of buffered peptone water with cysteine followed by selective enrichment in 10 mi of tetrathionate broth was the superior, resulting in the detection of S. enteritidis in all samples on six of the seven types of selective agar substrate investigated. This enrichment procedure also enabled detection of S. enteritidis in most of artificially inoculated shell egg and pasteurized liquid egg samples. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：395 / 399

页数：5

共 10 条

[1] A COMPARISON OF STANDARD CULTURAL METHODS FOR THE DETECTION OF FOODBORNE SALMONELLA [J].