Expression of cartilage-specific functional matrix chondromodulin-1 mRNA in rabbit growth plate chondrocytes and its responsiveness to growth stimuli in vitro

被引：32

作者：

Shukunami, C ^{[1
]}

Hiraki, Y ^{[1
]}

机构：

[1] Kyoto Univ, Inst Frontier Med Sci, Dept Mol Interact & Tissue Engn, Kyoto 6068507, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1998年 / 249卷 / 03期

基金：

日本学术振兴会; 日本科学技术振兴机构;

关键词：

D O I：

10.1006/bbrc.1998.9233

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Cartilage-specific functional matrix chondromodulin-I (ChM-I) is a 25 kDa glycoprotein purified from fetal bovine epiphyseal cartilage which stimulates the growth of rabbit chondrocytes and the colony formation of the cells in agarose. In the present study, we isolated rabbit ChM-I precursor cDNA by reverse transcription-polymerase chain reactions. Northern blot analysis revealed that the expression of ChM-I mRNA occurred in a tissue-specific manner in cartilage. Moreover, the ChM-I mRNA level was markedly changed in response to growth and differentiation stimuli in primary cultured chondrocytes. Among others, fibroblast growth factor-2, transforming growth factor-beta, and parathyroid hormone related peptide each markedly down-regulated the expression of ChM-I mRNA in chondrocytes, These results indicated that the expression ChM-I was placed under the dynamic control of local growth and differentiation factors. (C) 1998 Academic Press.

引用

页码：885 / 890

页数：6

共 32 条

[1] PARATHYROID HORMONE-RELATED PEPTIDE-DEPLETED MICE SHOW ABNORMAL EPIPHYSEAL CARTILAGE DEVELOPMENT ALTERED ENDOCHONDRAL BONE-FORMATION
AMIZUKA, N
WARSHAWSKY, H
HENDERSON, JE
GOLTZMAN, D
KARAPLIS, AC
[J]. JOURNAL OF CELL BIOLOGY, 1994, 126 (06) : 1611 - 1623
[2] TRANSFORMING GROWTH FACTOR-BETA CAUSES PARTIAL INHIBITION OF INTERLEUKIN-1 - STIMULATED CARTILAGE DEGRADATION INVITRO
ANDREWS, HJ
EDWARDS, TA
CAWSTON, TE
HAZLEMAN, BL
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1989, 162 (01) : 144 - 150
[3] Renal agenesis in mice homozygous for a gene trap mutation in the gene encoding heparan sulfate 2-sulfotransferase
Bullock, SL
Fletcher, JM
Beddington, RSP
Wilson, VA
[J]. GENES & DEVELOPMENT, 1998, 12 (12) : 1894 - 1906
[4] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[5] BASIC FIBROBLAST GROWTH-FACTOR (FGF) PROMOTES CARTILAGE REPAIR INVIVO
CUEVAS, P
BURGOS, J
BAIRD, A
[J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1988, 156 (02) : 611 - 618
[6] INDUCTION OF SWELLING, SYNOVIAL HYPERPLASIA AND CARTILAGE PROTEOGLYCAN LOSS UPON INTRAARTICULAR INJECTION OF TRANSFORMING GROWTH-FACTOR-BETA-2 IN THE RABBIT
ELFORD, PR
GRAEBER, M
OHTSU, H
AEBERHARD, M
LEGENDRE, B
WISHART, WL
MACKENZIE, AR
[J]. CYTOKINE, 1992, 4 (03) : 232 - 238
[7] FRENZ DA, 1994, DEVELOPMENT, V120, P415
[8] THE PRODUCTION OF TRANSFORMING GROWTH-FACTOR-BETA BY CHICK GROWTH PLATE CHONDROCYTES IN SHORT-TERM MONOLAYER-CULTURE
GELB, DE
ROSIER, RN
PUZAS, JE
[J]. ENDOCRINOLOGY, 1990, 127 (04) : 1941 - 1947
[9] DISTRIBUTION OF BASIC FIBROBLAST GROWTH-FACTOR IN THE 18-DAY RAT FETUS - LOCALIZATION IN THE BASEMENT-MEMBRANES OF DIVERSE TISSUES
GONZALEZ, AM
BUSCAGLIA, M
ONG, M
BAIRD, A
[J]. JOURNAL OF CELL BIOLOGY, 1990, 110 (03) : 753 - 765
[10] INDUCTION OF VASCULAR ENDOTHELIAL GROWTH-FACTOR EXPRESSION BY PROSTAGLANDIN E(2) AND E(1) IN OSTEOBLASTS
HARADA, S
NAGY, JA
SULLIVAN, KA
THOMAS, KA
ENDO, N
RODAN, GA
RODAN, SB
[J]. JOURNAL OF CLINICAL INVESTIGATION, 1994, 93 (06) : 2490 - 2496

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