The tightly regulated copper window in yeast

被引：61

作者：

Wegner, Seraphine V.

Sun, Fei

Hernandez, Nick

He, Chuan ^{[1
]}

机构：

[1] Univ Chicago, Dept Chem, Chicago, IL 60637 USA

来源：

CHEMICAL COMMUNICATIONS | 2011年 / 47卷 / 09期

关键词：

THIOLATE POLYNUCLEAR CLUSTER; TRANSCRIPTION FACTOR MAC1; DNA-BINDING DOMAIN; SACCHAROMYCES-CEREVISIAE; SUPEROXIDE-DISMUTASE; ACE1; PROTEIN; METALLOTHIONEIN; ZINC; SENSITIVITY;

D O I：

10.1039/c0cc04292g

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The two opposing yeast copper regulators, Ace1 and Mac1, were converted into two fluorescent probes, Ace1-FRET and Mac1-FRET, which selectively and sensitively respond to Cu+. The Cu+-binding curves obtained for both regulators as well as for the copper storage proteins Cup1 and Crs5 show that free copper(I) is maintained in a narrow window inside yeast.

引用

收藏

页码：2571 / 2573

页数：3

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