Gram-negative bacteria produce membrane vesicles which are capable of killing other bacteria

被引：227

作者：

Li, ZS ^{[1
]}

Clarke, AJ ^{[1
]}

Beveridge, TJ ^{[1
]}

机构：

[1] Univ Guelph, Coll Biol Sci, Dept Microbiol, Canadian Bacterial Dis Network, Guelph, ON N1G 2W1, Canada

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 20期

关键词：

D O I：

10.1128/JB.180.20.5478-5483.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Naturally produced membrane vesicles (MVs), isolated from 15 strains of gram-negative bacteria (Citrobacter, Enterobacter, Escherichia, Klebsiella, Morganella, Proteus, Salmonella, and Shigella strains), lysed many gram-positive (including Mycobacterium) and gram-negative cultures. Peptidoglycan zymograms suggested that MVs contained peptidoglycan hydrolases, and electron microscopy revealed that the murein sacculi were digested, confirming a previous modus operandi (J. L. Kadurugamuwa and T. J. Beveridge, J. Bacteriol. 174: 2767-2774, 1996). MV-sensitive bacteria possessed A1 alpha, A4 alpha, Al gamma, A2 alpha, and A4 gamma peptidoglycan chemotypes, whereas A3 alpha, A3 beta, A3 gamma, A4 beta, B1 alpha, and B1 beta chemotypes were not affected. Pseudomonas aeruginosa PAO1 vesicles possessed the most lytic activity.

引用

页码：5478 / 5483

页数：6

共 25 条

[1] ANALYSIS OF THE SODIUM DODECYL SULFATE-STABLE PEPTIDOGLYCAN AUTOLYSINS OF SELECT GRAM-NEGATIVE PATHOGENS BY USING RENATURING POLYACRYLAMIDE-GEL ELECTROPHORESIS [J].