Effect of postischaemic hypothermia on the mitochondrial damage induced by ischaemia and reperfusion in the gerbil

被引:26
作者
Canevari, L
Console, A
Tendi, EA
Clark, JB
Bates, TE
机构
[1] Inst Neurol, Dept Neurochem, London WC1N 3BG, England
[2] Catania Univ, Fac Med, Dept Biol Chem, Catania, Italy
基金
英国医学研究理事会;
关键词
mitochondria; cerebral ischemia; reperfusion; hypothermia; cytochrome c oxidase; respiratory chain;
D O I
10.1016/S0006-8993(98)01278-5
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
In order to test the effect of hypothermia on mitochondrial function damage following cerebral ischaemia/reperfusion, Mongolian gerbils were submitted to 30 min bilateral carotid occlusion and 2 h of reperfusion at 37 degrees C or 30 degrees C. After normothermic (37 degrees C) ischaemia/reperfusion, significant decreases in mitochondrial state 3 (+ ADP) oxygen consumption (-42.2%), complex II-III activity in synaptosomes (-31.7%) and complex IV were measured, in both free mitochondria and synaptosomes (-30.3% and -27.8% respectively). However, following hypothermic (30 degrees C) reperfusion, both respiration rates and all enzyme activities remained at levels not significantly different from those in the sham operated controls. (C) 1999 Elsevier Science B.V.
引用
收藏
页码:241 / 245
页数:5
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