Mutation of a single amino acid residue in the basic region of the Epstein-Barr virus (EBV) lytic cycle switch protein Zta (BZLFI) prevents reactivation of EBV from latency

被引：29

作者：

Schelcher, C

Valencia, S

Delecluse, HJ

Hicks, M

Sinclair, AJ ^{[1
]}

机构：

[1] Univ Sussex, Sch Life Sci, Dept Biochem, Brighton BN1 9QG, E Sussex, England

[2] German Canc Res Ctr, D-69120 Heidelberg, Germany

来源：

JOURNAL OF VIROLOGY | 2005年 / 79卷 / 21期

基金：

英国惠康基金;

关键词：

D O I：

10.1128/JVI.79.21.13822-13828.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Zta, the product of the BZLF1 gene carried by Epstein-Barr virus (EBV), is crucial for reactivation of EBV from latency. Zta is a member of the bZIP family of transcription factors, and in common with many of these, Zta possesses a conserved cysteine residue in its basic region (C189) and a further cysteine residue in its ZIP region (C222). We demonstrate that C189 is required to reactivate EBV from latency but C222 is not and that this single amino acid affects two independent functions of Zta, (i) binding to a Zta-responsive site and (ii) manipulating the cell cycle.

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页码：13822 / 13828

页数：7

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