An immunoenzymatic procedure for human apo B-containing particles quantification using monoclonal antibodies

被引：5

作者：

Bustos, P ^{[1
]}

Calvo, C ^{[1
]}

Ulloa, N ^{[1
]}

Quiroga, A ^{[1
]}

Sepulveda, J ^{[1
]}

机构：

[1] Univ Concepcion, Fac Farm, Dept Bioquim Clin & Immunol, Concepcion, Chile

来源：

HYBRIDOMA | 1998年 / 17卷 / 04期

关键词：

D O I：

10.1089/hyb.1998.17.395

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The present report describes a new monoclonal antibody-based enzyme immunoassay (ELISA) for the quantification of apolipoprotein (apo) B-containing particles in plasma. Native low-density lipoprotein (LDL) and a reference serum were utilized to prepare the standard curve. Three different antibodies to apo B-100-4A6E3, 6A10B10, and 2D9-all produced in our laboratory, were examined. The apparent affinity constants of the monoclonal antibodies (MAbs) 4A6E3, 6A10B10, and 2D9 were 2.9 x 10(9), 1.74 x 10(9), and 0.63 x 10(8), respectively. The standard curve was generated for an apo B-LDL range of 0.1 to 4.0 mu g/ml. Evaluating the concentration of apo B-containing particles in plasma may allow for a more accurate assessment of the risk of coronary artery disease.

引用

页码：395 / 401

页数：7

共 19 条

[1] RELATIONSHIP BETWEEN THE SIZE AND PHOSPHOLIPID CONTENT OF LOW-DENSITY LIPOPROTEINS [J].

BARTER, PJ ;

RAJARAM, OV ;

LIANG, HQ ;

RYE, KA .

BIOCHIMICA ET BIOPHYSICA ACTA, 1993, 1166 (01) :135-137

[2] Development of monoclonal antibodies for the selective isolation of human plasma apolipoprotein A-containing particles [J].

Calvo, C ;

Bustos, P ;

Sepulveda, J ;

Ulloa, N ;

Sepulveda, V .

HYBRIDOMA, 1995, 14 (06) :603-608

[3]

CHAN L, 1992, J BIOL CHEM, V267, P25621

[4] Characteristics of ten charge-differing subfractions isolated from human native low-density lipoproteins (LDL). No evidence of peroxidative modifications [J].

Chappey, B ;

Myara, I ;

Benoit, MO ;

Maziere, C ;

Maziere, JC ;

Moatti, N .

BIOCHIMICA ET BIOPHYSICA ACTA-LIPIDS AND LIPID METABOLISM, 1995, 1259 (03) :261-270

[5]

CURTISS LK, 1982, J BIOL CHEM, V257, P5213

[6]

DORMANS TPJ, 1991, CLIN CHEM, V37, P853

[7] RELATION OF ARTERIOGRAPHICALLY DEFINED CORONARY-ARTERY DISEASE TO SERUM-LIPOPROTEIN PARTICLES MAPPED WITH MONOCLONAL-ANTIBODIES [J].

FIEVET, C ;

NUTTENS, MC ;

DUCIMETIERE, P ;

FRUCHART, JC ;

BERTRAND, M ;

SALOMEZ, JL .

CIRCULATION, 1991, 84 (01) :153-159

[8] PLASMA APOLIPOPROTEIN A-I, A-II, B, E AND C-III CONTAINING PARTICLES IN MEN WITH PREMATURE CORONARY-ARTERY DISEASE [J].

GENEST, JJ ;

BARD, JM ;

FRUCHART, JC ;

ORDOVAS, JM ;

WILSON, PFW ;

SCHAEFER, EJ .

ATHEROSCLEROSIS, 1991, 90 (2-3) :149-157

[9] DISTRIBUTION AND CHEMICAL COMPOSITION OF ULTRACENTRIFUGALLY SEPARATED LIPOPROTEINS IN HUMAN SERUM [J].

HAVEL, RJ ;

EDER, HA ;

BRAGDON, JH .

JOURNAL OF CLINICAL INVESTIGATION, 1955, 34 (09) :1345-1353

[10] CONTINUOUS CULTURES OF FUSED CELLS SECRETING ANTIBODY OF PREDEFINED SPECIFICITY [J].

KOHLER, G ;

MILSTEIN, C .

NATURE, 1975, 256 (5517) :495-497

← 1 2 →