Massive release of extracellular vesicles from cancer cells after photodynamic treatment or chemotherapy

被引：111

作者：

Aubertin, Kelly ^{[1
,2
]}

Silva, Amanda K. A. ^{[1
,2
]}

Luciani, Nathalie ^{[1
,2
]}

Espinosa, Ana ^{[1
,2
]}

Djemat, Aurelie ^{[3
]}

Charue, Dominique ^{[4
,5
]}

Gallet, Francois ^{[1
,2
]}

Blanc-Brude, Olivier ^{[4
,5
]}

Wilhelm, Claire ^{[1
,2
]}

机构：

[1] CNRS, UMR 7057, Lab Mat & Syst Complexes, 10 Rue Alice Domon & Leonie Duquet, F-75205 Paris 13, France

[2] Univ Paris Diderot, 10 Rue Alice Domon & Leonie Duquet, F-75205 Paris 13, France

[3] Univ Paris Diderot, UMR CNRS 7592, Animalerie BUFFON, Inst Jacques Monod, F-75205 Paris 13, France

[4] Univ Paris 05, PRES Sorbonne Paris Cite, ParCC Paris Cardiovasc Ctr, INSERM UMRs 970, 56 Rue Leblanc, F-75908 Paris 15, France

[5] Hop Europeen Georges Pompidou, AP HP, 56 Rue Leblanc, F-75908 Paris 15, France

来源：

SCIENTIFIC REPORTS | 2016年 / 6卷

关键词：

MEMBRANE-VESICLES; THERAPY RESPONSIVENESS; TUMOR-CELLS; MICROVESICLES; MICROPARTICLES; DOXORUBICIN; EXOSOMES; INFLAMMATION; ADRIAMYCIN; EXPRESSION;

D O I：

10.1038/srep35376

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

070301 [无机化学]; 070403 [天体物理学]; 070507 [自然资源与国土空间规划学]; 090105 [作物生产系统与生态工程];

摘要：

Photodynamic therapy is an emerging cancer treatment that is particularly adapted for localized malignant tumor. The phototherapeutic agent is generally injected in the bloodstream and circulates in the whole organism as a chemotherapeutic agent, but needs light triggering to induce localized therapeutic effects. We found that one of the responses of in vitro and in vivo cancer cells to photodynamic therapy was a massive production and emission of extracellular vesicles (EVs): only 1 hour after the photo-activation, thousands of vesicles per cell were emitted in the extracellular medium. A similar effect has been found after treatment with Doxorubicin (chemotherapy), but far less EVs were produced, even 24 hours after the treatment. Furthermore, we found that the released EVs could transfer extracellular membrane components, drugs and even large intracellular objects to naive target cells. In vivo, photodynamic treatment and chemotherapy increased the levels of circulating EVs several fold, confirming the vast induction of cancer cell vesiculation triggered by anti-cancer therapies.

引用

页数：11

共 53 条

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