Ethanol production from dilute acid hydrolysate of rice hulls using genetically engineered Escherichia coli

被引:47
作者
Moniruzzaman, M [1 ]
Ingram, LO [1 ]
机构
[1] Univ Florida, Inst Food & Agr Sci, Dept Microbiol & Cell Sci, Gainesville, FL 32611 USA
关键词
ethanol; rice hulls; E-coli; starch; hemicellulose;
D O I
10.1023/A:1005490501736
中图分类号
Q81 [生物工程学(生物技术)]; Q93 [微生物学];
学科分类号
071005 ; 0836 ; 090102 ; 100705 ;
摘要
Approximately 30% of rice hulls, which represent an abundant residue with little commercial value, was solubilized with 0.4 M H2SO4 acid to produce a syrup containing over 100 g monomer sugar/l. Toxins generated during hydrolysis were mitigated with Ca(OH)(2). Treated hydrolysate plus additional nutrients was fermented with Escherichia coli KO11 to produce over 46 g ethanol/L in 72 h (92% of theoretical yield).
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页码:943 / 947
页数:5
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