The RP-HPLC analysis of anthocyanins

被引：12

作者：

Luczkiewicz, M ^{[1
]}

Cisowski, W ^{[1
]}

机构：

[1] Med Univ Gdansk, Dept Pharmacognosy, PL-80416 Gdansk, Poland

来源：

CHROMATOGRAPHIA | 1998年 / 48卷 / 5-6期

关键词：

column liquid chromatography; acylated anthocyanins; Rudbeckia hirta L; in vitro cultures;

D O I：

10.1007/BF02467704

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Conditions were determined for the separation of a complex set of anthocyanins (free aglycones, mono- and multiglycosides and esterified forms) by HPLC. The optimised gradient elution method was then used to carry out qualitative and quantitative analysis of anthocyanin compounds present in the callus tissue of Rudbeckia hirta L. and the tubular flowers of the soil-based plant. The summary content of anthocyanin pigments and the content of the main pigment was identified in the analysed biomass. The method developed is useful for the purposes of monitoring the process of biosynthesis of anthocyanins in tissues obtained through in vitro cultures. The advantages of the method for anthocyanins and its application to other anthocyanin-rich materials are also discussed.

引用

页码：360 / 364

页数：5

共 15 条

[1] SIMULTANEOUS ANALYSIS OF ANTHOCYANINS AND ANTHOCYANIDINS ON CELLULOSE THIN-LAYERS [J].