Regional-specific global cytosine methylation and DNA methyltransferase expression in the adult rat hippocampus

被引：57

作者：

Brown, Shelley E. ^{[2
]}

Weaver, Ian C. G. ^{[1
]}

Meaney, Michael J. ^{[1
]}

Szyf, Moshe ^{[2
]}

机构：

[1] Douglas Hosp, Res Ctr, Verdun, PQ H4H 1R3, Canada

[2] McGill Univ, Dept Pharmacol & Therapeut, Montreal, PQ H3G 1Y6, Canada

来源：

NEUROSCIENCE LETTERS | 2008年 / 440卷 / 01期

基金：

加拿大健康研究院;

关键词：

DNMT-1; DNMT-3a/b; DNA methylation; hippocampus; maternal care;

D O I：

10.1016/j.neulet.2008.05.028

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

Recent observations suggest that DNA methylation plays an important role in memory and long-term potentiation (LTP) in the hippocampus and is involved in programming the offspring epigenome in response to maternal care. Global DNA methylation is believed to be stable postnatally and to be similar across tissues in the adult mammal. It has also been a long held belief that DNA methyltransferases (DNMTs) play a very limited role in postmitotic tissues. Recent data suggests a more dynamic role for DNA methylation in the brain postnatally, therefore we examined the global state of methylation and the expression of the known DNMTs in the different regions of the hippocampus. We observed strikingly different levels of global methylation in the adult rat dentarte gyrus (DG) and CA1 region in comparison with the CA2 and CA3 regions. mRNA levels of DNA methyltransferases exhibited similar regional specificity and were correlated with global DNA methylation levels. These regional differences in global methylation and expression of the DNA methylation machinery in the adult brain are consistent with the emerging hypothesis that DNA methylation may play a dynamic physiological role in the adult brain. Crown Copyright (C) 2008 Published by Elsevier Ireland Ltd. All rights reserved.

引用

页码：49 / 53

页数：5

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