Pyrosequencing as a method for grouping of Listeria monocytogenes strains on the basis of single-nucleotide polymorphisms in the inlB gene

被引：22

作者：

Unnerstad, H

Ericsson, H

Alderborn, A

Tham, W

Danielsson-Tham, ML

Mattsson, JG

机构：

[1] Swedish Univ Agr Sci, Fac Vet Sci, Dept Food Hyg, SE-75007 Uppsala, Sweden

[2] Pyrosequencing AB, SE-75228 Uppsala, Sweden

[3] Natl Vet Inst, Dept Parasitol, SWEPAR, SE-75189 Uppsala, Sweden

来源：

APPLIED AND ENVIRONMENTAL MICROBIOLOGY | 2001年 / 67卷 / 11期

关键词：

D O I：

10.1128/AEM.67.11.5339-5342.2001

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

By using pyrosequencing (i.e., sequencing by synthesis) 106 strains of different serovars of Listeria monocytogenes were rapidly grouped into four categories based on nucleotide variations at positions 1575 and 1578 of the inlB gene. Strains of serovars 1/2a and 1/2c constituted one group, and strains of serovars 1/2b and 3b constituted another group, whereas serovar 4b strains were separated into two groups.

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页码：5339 / 5342

页数：4

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