Activation-tagged tobacco mutants that are tolerant to antimicrotubular herbicides are cross-resistant to chilling stress

被引：25

作者：

Ahad, A ^{[1
]}

Wolf, J ^{[1
]}

Nick, P ^{[1
]}

机构：

[1] Inst Biol 2, D-79104 Freiburg, Germany

来源：

TRANSGENIC RESEARCH | 2003年 / 12卷 / 05期

关键词：

activation tagging; antimicrotubular herbicides; aryl carbamates; chilling tolerance; microtubules; tyrosinylated tubulin;

D O I：

10.1023/A:1025814814823

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

DNA activation tagging was used to generate tobacco mutants with increased tolerance to antimicrotubular herbicides and chilling stress. After transformation, protoplast-derived calli were screened for tolerance to treatments that affect microtubule assembly. In one screen mutants with tolerance to aryl carbamates ( a blocker of microtubule assembly) were selected, the second screen was targeted to chilling-tolerant mutants that could survive for several months at 3degreesC, a third screen combined both factors. The resistance of these mutants to aryl carbamates or chilling was accompanied by resistance of microtubules to these factors. The carbamate tolerant mutants were cross-resistant to chilling stress. This was mirrored by an adaptive reorganization of microtubules and a reduction of microtubule dynamics in response to chilling. The analysis of these mutants suggests ( 1) that microtubule dynamics limit the tolerance to chilling and EPC, and ( 2) that the cold sensitivity of microtubules limits chilling tolerance in tobacco.

引用

页码：615 / 629

页数：15

共 49 条

[1] Suppression of endogenous α and β tubulin synthesis in transgenic maize calli overexpressing α and β tubulins [J].