A newly developed adenovirus-mediated transfer of a wild-type p53 gene increases sensitivity to cis-diamminedichloroplatinum (II) in p53-deleted ovarian cancer cells

被引：28

作者：

Kanamori, Y

Kigawa, J

Minagawa, Y

Irie, T

Oishi, T

Shimada, M

Takahashi, M

Nakamura, T

Sato, K

Terakawa, N

机构：

[1] Tottori Univ, Sch Med, Dept Obstet & Gynaecol, Yonago, Tottori 683, Japan

[2] Tottori Univ, Sch Med, Dept Biol Mol, Yonago, Tottori 683, Japan

来源：

EUROPEAN JOURNAL OF CANCER | 1998年 / 34卷 / 11期

关键词：

ovarian cancer; CDDP; p53 gene transfer; recombinant adenovirus;

D O I：

10.1016/S0959-8049(98)00199-3

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

A new recombinant adenovirus carrying a wild-type p53 gene (AxCAp53) was developed and the combination effect of p53 gene transfer and cis-diamminedichloroplatinum (II) (CDDP) was examined in an ovarian cancer cell line, SK-OV-3, with deletion of the p53 gene. AxCAp53 showed a high efficiency of gene transduction and increased sensitivity to CDDP in the SK-OV-3 cells. It was found that the sensitivity of the cells to CDDP correlated with the amount of infectious units of virus per cell of AxCAp53 which correlated with p53 protein expression. The results suggest that the combination of CDDP and AxCAp53 may be a potential strategy for the therapy of CDDP-resistant ovarian cancer. (C) 1998 Elsevier Science Ltd. All rights reserved.

引用

页码：1802 / 1806

页数：5

共 23 条

[1] BRADFORD MM, 1976, ANAL BIOCHEM, V72, P248, DOI 10.1016/0003-2697(76)90527-3
[2] FINNEY DJ, 1971, METHOD INFORM MED, V10, P237
[3] FRITSCHE M, 1993, ONCOGENE, V8, P307
[4] FUJIWARA T, 1994, CANCER RES, V54, P2287
[5] FUKUCHI K, 1994, IN VITRO CELL DEV-AN, V30A, P300
[6] Hamada K, 1996, CANCER RES, V56, P3047
[7] JONATHAN J, 1996, CANCER RES, V56, P2178
[8] A SIMPLE AND EFFICIENT METHOD FOR PURIFICATION OF INFECTIOUS RECOMBINANT ADENOVIRUS
KANEGAE, Y
MAKIMURA, M
SAITO, I
[J]. JAPANESE JOURNAL OF MEDICAL SCIENCE & BIOLOGY, 1994, 47 (03): : 157 - 166
[9] P53, CELL-CYCLE CONTROL AND APOPTOSIS - IMPLICATIONS FOR CANCER
KASTAN, MB
CANMAN, CE
LEONARD, CJ
[J]. CANCER AND METASTASIS REVIEWS, 1995, 14 (01) : 3 - 15
[10] KELLEN JA, 1994, ANTICANCER RES, V14, P433

← 1 2 3 →