Differential roles for IL-15R α-chain in NK cell development and Ly-49 induction

被引：45

作者：

Kawamura, T

Koka, R

Ma, A

Kumar, V

机构：

[1] Univ Chicago, Dept Pathol, Chicago, IL 60637 USA

[2] Univ Chicago, Dept Med, Chicago, IL 60637 USA

来源：

JOURNAL OF IMMUNOLOGY | 2003年 / 171卷 / 10期

关键词：

D O I：

10.4049/jimmunol.171.10.5085

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

IL-15Ralpha-deficient (IL-15Ralpha(-/-)) mice lack NK cells. However, when bone marrow (BM) progenitors from IL-15Ralpha(-/-) mice were cultured with IL-7, stem cell factor and 1143 ligand, followed by IL-15, they were able to differentiate into functional NK cells, indicating that IL-15Ralpha is not critical for NK cell development. Whereas NK cells generated in vitro from IL-15Ralpha(-/-) BM progenitors expressed CD94/NKG2, they failed to express Ly-49 receptors. In keeping with this, when IL-15Ralpha(-/-) BM cells were transferred into wild type recipients, they gave rise to NK cells in vivo, but with greatly reduced expression of Ly-49 receptors. Furthermore, the small numbers of NK cells found in IL-15(-/-) as well as IL-15Ralpha(-/-) but not flt3 ligand(-/-) mice expressed much lower levels of Ly-49 receptors than those from wild type mice. These results indicate a novel role for IL-15Ralpha-chain in Ly-49 induction on developing NK cells.

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页码：5085 / 5090

页数：6

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