A modified HPLC method for the determination of vancomycin in plasma and tissues and comparison to FPIA (TDX)

被引：79

作者：

Farin, D ^{[1
]}

Piva, GA ^{[1
]}

Gozlan, I ^{[1
]}

Kitzes-Cohen, R ^{[1
]}

机构：

[1] Technion Israel Inst Technol, Rappaport Fac Med, Carmel Med Ctr, Clin Pharmacol & Infect Dis Unit, IL-34362 Haifa, Israel

来源：

JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS | 1998年 / 18卷 / 03期

关键词：

vancomycin; reversed phase liquid chromatography; TDX; plasma; tissues;

D O I：

10.1016/S0731-7085(98)00095-8

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

A modified high performance liquid chromatography (HPLC) method for the quantification of vancomycin levels in plasma and tissues is described. The method uses solid phase extraction (SPE) of vancomycin from the samples and reversed phase HPLC with UV detection. The method was fully validated in terms of recovery, linearity, selectivity and various stability conditions. Vancomycin was determined in plasma samples obtained from 15 patients undergoing cardiopulmonary bypass, before and repeatedly during 12 h after drug administration. The vancomycin levels in plasma were measured by HPLC and by fluorescence polarization immunoassay (FPIA) (TDX). The following correlation was found: TDX=0.84 HPLC+1.04. The mean vancomycin levels in skin, fat, atrium, pericardium and sternum, before and after bypass, are reported. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：367 / 372

页数：6

共 16 条

[1] DETERMINATION OF VANCOMYCIN IN SERUM AND TISSUES BY COLUMN LIQUID-CHROMATOGRAPHY USING SOLID-PHASE EXTRACTION [J].