Prognostic impact of CD45 antigen expression in high-risk, childhood B-cell precursor acute lymphoblastic leukemia

被引：15

作者：

Nakamura, A ^{[1
]}

Tsurusawa, M ^{[1
]}

Kato, A ^{[1
]}

Taga, T ^{[1
]}

Hatae, YH ^{[1
]}

Miyake, M ^{[1
]}

Mimaya, J ^{[1
]}

Onodera, N ^{[1
]}

Watanabe, A ^{[1
]}

Watanabe, T ^{[1
]}

Kanegane, H ^{[1
]}

Matsushita, T ^{[1
]}

Iwai, A ^{[1
]}

Hyakuna, N ^{[1
]}

Gushi, K ^{[1
]}

Kawakami, T ^{[1
]}

Sekine, I ^{[1
]}

Izichi, O ^{[1
]}

Asami, K ^{[1
]}

Kikuta, AH ^{[1
]}

Tanaka, A ^{[1
]}

Fujimoto, T ^{[1
]}

机构：

[1] Aichi Med Univ, Dept Pediat, Aichi 4801195, Japan

来源：

LEUKEMIA & LYMPHOMA | 2001年 / 42卷 / 03期

关键词：

childhood; acute lymphoblastic leukemia; prognosis;

D O I：

10.3109/10428190109064596

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

To evaluate the clinical implications of CD45 expression in acute childhood lymphoblastic leukemia (ALL), we measured the CD45 expression of blast cells from 133 untreated patients with childhood B-precursor ALL (n=118) or T-ALL (n=15). CD45 expression ( greater than or equal to 20%) was detected in all 15 cases (100%) of T-ALL, and 101 cases (86%) of B-precursor ALL. In 122 cases, the fluorescence intensity of the CD45 expression was measured as a relative value; the ratio of average linear values (RALV) of CD45 on the blasts to that on CD3-positive T-lymphocytes from the same specimen. The expression was more intense in the T-ALL cases than in the B-precursor ALL cases (RALV, mean +/- SE: T-ALL 0.230 +/-0.04 vs. pro-B ALL 0.150 +/-0.012 / pre-B ALL 0.153 +/-0.019, p <0.05). However, the intensity of the CD10, CD19, CD20 and CD34 antigen immunoreactivity did not correlate with the CD45 expression. Patients with hyperdiploidy (chromosome number > 50) showed significantly lower levels of CD45 expression than patients with t(1;19) or normal karyotypies (RALV, mean +/- SE: 0.081 +/-0.022 vs. 0.133 +/-0.03/ 0.143 +/-0.019, p <0.05). Other clinical features such as age, gender and WBC count did not correlate with CD45 expression. The prognostic implications of CD45 expression were studied in non-high-risk (low-risk + intermediate-risk) (n=60) and high-risk patients (n=52) with B-precursor ALL who had been treated with the risk-directed protocol of ALL-941 trial. Although CD45 expression did not correlate with the event-free survival (EFS) of the non-high-risk patients, there was a significant correlation between the expression levels and the EFS of the high-risk patients: the 3-year EFS rate of the CD45(low) group (n=26, RALV=0.017- 0.132) was 88 +/-7% versus the CD45(high) group (n=26, RALV=0.133 - 0.450) at 34 +/- 24% (p <0.05). These results show that the levels of expression of the CD45 antigen on leukemic lymphoblasts are significantly correlated with the clinical features and prognosis of childhood ALL.

引用

页码：393 / 398

页数：6

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