Chemical composition of volatile extract and biological activities of volatile and less-volatile extracts of juniper berry (Juniperus drupacea L.) fruit

被引:34
作者
El-Ghorab, Ahmed [2 ]
Shaaban, Hamdy A. [2 ]
El-Massry, Khaled F. [2 ]
Shibamoto, Takayuki [1 ]
机构
[1] Univ Calif Davis, Dept Environm Toxicol, Davis, CA 95616 USA
[2] Natl Res Ctr, Flavor & Aroma Dept, Cairo, Egypt
关键词
alpha-pinene; antimicrobial activity; antioxidant activity; camphor; juniper berry fruit; volatile chemicals;
D O I
10.1021/jf8001747
中图分类号
S [农业科学];
学科分类号
09 [农学];
摘要
Volatile chemicals in a dichloromethane extract from a steam distillate of juniper berry fruit (Juniperus drupacea L.) and its two column chromatographic fractions (eluted with hexane and ethyl ether) were analyzed by gas chromatography/mass spectrometry. The major compounds in the dichloromethane extract were a-pinene (23.73%), thymol methyl ether (17.32%), and camphor (10.12%). A fraction eluted with hexane contained a-pinene (44.24%) as the major constituent. A fraction eluted with ethyl ether had thymol methyl ether (22.27%) and camphor (19.65%) as the main components. Three samples prepared from the distillate and two additional samples prepared by petroleum ether and ethanol extraction directly from juniper berry fruits exhibited clear antioxidant activities with dose response in both 1,2-diphenyl picrylhydrazyl and beta-carotene assays. All samples except the hexane fraction showed comparable activities to that of the synthetic antioxidant t-butyl hydroquinone at a level of 200 mu g/mL in the two testing systems. The extracts of dichloromethane, petroleum ether, and ethanol exhibited appreciable antimicrobial activities against six microorganisms with minimum inhibitory concentrations ranging from 0.5 mg/mL (volatile extract against Candida albicans) to 1.2 mg/mL (ethanol extract against Aspergillus niger). The results of the present study suggest that this fruit could be a natural antioxidant supplement for foods and beverages.
引用
收藏
页码:5021 / 5025
页数:5
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