Use of PCR analyses to define the distribution of Ralstonia species recovered from patients with cystic fibrosis

被引：51

作者：

Coenye, T

Spilker, T

Reik, R

Vandamme, P

LiPuma, JJ

机构：

[1] Univ Michigan, Sch Med, Dept Pediat & Communicable Dis, Ann Arbor, MI 48109 USA

[2] State Univ Ghent, Fac Sci, Microbiol Lab, B-9000 Ghent, Belgium

来源：

JOURNAL OF CLINICAL MICROBIOLOGY | 2005年 / 43卷 / 07期

关键词：

D O I：

10.1128/JCM.43.7.3463-3466.2005

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Two new PCR assays (for Ralstonia species and Ralstonia respiraculi), together with previously published PCR assays, were used to assess Ralstonia isolates recovered from 111 cystic fibrosis patients. Ralstonia mannitolilytica accounted for 46% of isolates, while R. respiraculi and Ralstonia pickettii accounted for 19% and 18%, respectively. Ralstonia basilensis and Ralstonia metallidurans, species not previously recovered from human samples, were also identified.

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页码：3463 / 3466

页数：4

相关论文

共 21 条

[1]

[Anonymous], CHEM METHODS PROKARY

[2] Microbiology of sputum from patients at cystic fibrosis centers in the United States [J].

Burns, JL ;

Emerson, J ;

Stapp, JR ;

Yim, DL ;

Krzewinski, J ;

Louden, L ;

Ramsey, BW ;

Clausen, CR .

CLINICAL INFECTIOUS DISEASES, 1998, 27 (01) :158-163

[3] Burkholderia ambifaria sp nov., a novel member of the Burkholderia cepacia complex including biocontrol and cystic fibrosis-related isolates [J].

Coenye, T ;

Mahenthiralingam, E ;

Henry, D ;

LiPuma, JJ ;

Laevens, S ;

Gillis, M ;

Speert, DP ;

Vandamme, P .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :1481-1490

[4] Ralstonia respiraculi sp nov., isolated from the respiratory tract of cystic fibrosis patients [J].

Coenye, T ;

Vandamme, P ;

LiPuma, JJ .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2003, 53 :1339-1342

[5] Infection by Ralstonia species in cystic fibrosis patients:: Identification of R-pickettii and R-mannitolilytica by polymerase chain reaction [J].

Coenye, T ;

Vandamme, P ;

LiPuma, JJ .

EMERGING INFECTIOUS DISEASES, 2002, 8 (07) :692-696

[6] Characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of Inquilinus limosus gen. nov., sp nov. [J].

Coenye, T ;

Goris, J ;

Spilker, T ;

Vandamme, P ;

LiPuma, JJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (06) :2062-2069

[7] Identification of Pandoraea species by 16S ribosomal DNA-based PCR assays [J].

Coenye, T ;

Liu, LX ;

Vandamme, P ;

LiPuma, JJ .

JOURNAL OF CLINICAL MICROBIOLOGY, 2001, 39 (12) :4452-4455

[8] Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp nov. [J].

Coenye, T ;

Goris, J ;

De Vos, P ;

Vandamme, P ;

LiPuma, JJ .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2003, 53 :1075-1080

[9] Classification of Ralstonia pickettii biovar 3/'thomasii' strains (Pickett 1994) and of new isolates related to nosocomial recurrent meningitis as Ralstonia mannitolytica sp nov. [J].

De Baere, T ;

Steyaert, S ;

Wauters, G ;

De Vos, P ;

Goris, J ;

Coenye, T ;

Suyama, T ;

Verschraegen, G ;

Vaneechoutte, M .

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY, 2001, 51 :547-558

[10] Microbiological and clinical aspects of infection associated with Stenotrophomonas maltophilia [J].

Denton, M ;

Kerr, KG .

CLINICAL MICROBIOLOGY REVIEWS, 1998, 11 (01) :57-+