Anti-endotoxin antibodies directed against Escherichia coli R-1 oligosaccharide core tetanus toxoid conjugate bind to smooth, live bacteria and smooth lipopolysaccharides and attenuate their tumor necrosis factor stimulating activity

被引：15

作者：

Lugowski, C

Niedziela, T

Jachymek, W

机构：

[1] Ludwik Hirszfeld Inst. of I., 53-114 Wrocław

来源：

FEMS IMMUNOLOGY AND MEDICAL MICROBIOLOGY | 1996年 / 16卷 / 01期

关键词：

endotoxin; core region; neoglycoconjugate; Escherichia coli; antibody; tumor necrosis factor;

D O I：

10.1016/S0928-8244(96)00067-3

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

The reactivity of anti-OS R1-tetanus toroid serum with various smooth and rough lipopolysaccharides have been determined in enzyme-linked immunosorbent assay (ELISA) and with intact, live smooth bacterial cells in flow cytometry. All tested smooth lipopolysaccharides of R1 core type belonging to nine different O-serotypes reacted strongly with anti-conjugate antibodies. Flow cytometry showed that anti-core antibodies labelled more than 95% of bacterial cells with high intensity of fluorescence. The anti-conjugate serum was able to react with free form of smooth lipopolysaccharides and to inhibit their TNF stimulation activity in in vitro and in vivo models.

引用

页码：31 / 38

页数：8

共 14 条

[1] A HIGHLY SENSITIVE CELL-LINE, WEHI-164 CLONE 13, FOR MEASURING CYTOTOXIC FACTOR TUMOR-NECROSIS-FACTOR FROM HUMAN-MONOCYTES [J].