A comparative metabolomic study of NHR-49 in Caenorhabditis elegans and PPAR-α in the mouse

被引：63

作者：

Atherton, Helen J. ^{[1
]}

Jones, Oliver A. H. ^{[1
]}

Malik, Shahid ^{[2
]}

Miska, Eric A. ^{[3
]}

Griffin, Julian L. ^{[1
]}

机构：

[1] Univ Cambridge, Dept Biochem, Cambridge CB2 1QW, England

[2] Chenomx, Edmonton, AB T5K 2J1, Canada

[3] Univ Cambridge, Canc Res UK Gurdon Inst, Wellcome Trust, Cambridge CB2 1QN, England

来源：

FEBS LETTERS | 2008年 / 582卷 / 12期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

peroxisome proliferator-activated receptors; functional genomics; nuclear hormone receptors; NMR spectroscopy; gas chromatography mass spectrometry;

D O I：

10.1016/j.febslet.2008.04.020

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Proton Nuclear Magnetic Resonance spectroscopy and Gas Chromatography Mass Spectrometry based metabolomics has been used in conjunction with multivariate statistics to examine the metabolic changes in Caenorhabditis elegans following the deletion of nuclear hormone receptor-49 (nhr-49). Deletion of the receptor produced profound changes in fatty acid metabolism, in particular an increase in the ratio of unsaturated to saturated fatty acids, a decrease in the concentration of glucose and increases in lactate and alanine. Given the proposed functional similarity between nhr-49 and the mammalian peroxisome proliferator-activated receptors (PPARs) these changes were compared with the metabolome of the PPAR-alpha null mouse. The metabolomic approach demonstrated a number of similarities including the regulation of lipid synthesis, beta-oxidation of fatty acids and changes in glycolysis/gluconeogenesis. (C) 2008 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.

引用

页码：1661 / 1666

页数：6

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