Ultratrace determination of adenine in the presence of copper by adsorptive stripping voltammetry

被引：44

作者：

Farias, PAM ^{[1
]}

Wagener, AD ^{[1
]}

Castro, AA ^{[1
]}

机构：

[1] Pontificia Univ Catolica Rio de Janeiro, Dept Chem, BR-22453900 Rio De Janeiro, Brazil

来源：

TALANTA | 2001年 / 55卷 / 02期

关键词：

adenine determination; ssDNA; guanine; thymine; cytosine; copper; stripping voltammetry;

D O I：

10.1016/S0039-9140(01)00422-2

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

An electrochemical stripping procedure for ultra-trace measurements of adenine is described based on the adsorption of the adenine-copper complex on a static mercury drop electrode. Cyclic voltammetry was used to characterize the interfacial and redox behavior. Optimum experimental conditions were found when using a 0.005 M NaOH solution containing 0.4 ppm of copper, an accumulation potential of -0.30 V, a scan rate of 100 mV s(-1) and a linear scan mode. There is a linear response to adenine concentration in the range of 0.1-1.0 ppb and the detection limit for 6 min accumulation time was of 4.0 ppt (3.0 x 10(-11) M). Proper conditions for measuring the adenine in presence of guanine, thymine and cytosine were also investigated. The method was applied for the determination of adenine in a sample of single-stranded calf thymus DNA. (C) 2001 Elsevier Science B.V. All rights reserved.

引用

页码：281 / 290

页数：10

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