Reference-compensated biosensing using a dual-channel surface plasmon resonance sensor system based on a planar lightpipe configuration

被引：53

作者：

Nenninger, GG

Clendenning, JB

Furlong, CE

Yee, SS ^{[1
]}

机构：

[1] Univ Washington, Dept Elect Engn, Seattle, WA 98195 USA

[2] Univ Washington, Dept Med, Seattle, WA 98195 USA

[3] Univ Washington, Dept Genet, Seattle, WA 98195 USA

来源：

SENSORS AND ACTUATORS B-CHEMICAL | 1998年 / 51卷 / 1-3期

关键词：

optical sensors; surface plasmon resonance; refractive index; lightpipe;

D O I：

10.1016/S0925-4005(98)00218-4

中图分类号：

O65 [分析化学];

学科分类号：

070302 ; 081704 ;

摘要：

We present the design and experimental verification for a surface plasmon resonance (SPR) bio-sensor system using a second sensor channel for reference compensation during antibody-antigen binding experiments. Using a lightpipe configuration SPR sensor with two sensor surfaces and flow cell volumes, we demonstrate the use of the second sensor channel, with functionalisation different than the primary sensor channel, to compensate for changes in the refractive index of the bulk solution caused by analyte concentration or temperature differences. (C) 1998 Elsevier Science S.A. All rights reserved.

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页码：38 / 45

页数：8

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