Fibroblast contraction of a collagen-GAG matrix

被引:106
作者
Freyman, TM
Yannas, IV
Yokoo, R
Gibson, LJ
机构
[1] MIT, Dept Mat Sci & Engn, Cambridge, MA 02139 USA
[2] MIT, Dept Mech Engn, Cambridge, MA 02139 USA
关键词
contraction; collagen; fibroblast; force;
D O I
10.1016/S0142-9612(01)00034-5
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
Contractile cells, found in wounded or diseased tissues, are associated with the formation of scar tissue. The complexity of contraction in vivo has led to the development of models of contraction by cells in vitro. In this work, a device was developed which quantitatively measured the contractile force developed by fibroblasts seeded into a collagen-glycosaminoglycan porous matrix in vitro. This device differed from most of those previously described in that it directly transferred cellular contractile force to the force transducer (a cantilever beam) and that it used a porous matrix rather than a collagen gel. The data for the increase in contractile force with time were fit to a mathematical equation using two fitting parameters. Data were then compared using the fitting parameters and the cell density. A study of the effect of cell density on the contractile force resulted in a linearly proportional relationship. Subsequent normalization of force by cell density or number resulted in a value of contractile force per cell, I nN, that was independent of cell density. The time for the contractile force to develop was also independent of cell density. These results suggest that, in this system, cells develop contractile force individually, irrespective of the force generated by surrounding cells. (C) 2001 Elsevier Science Ltd. All rights reserved.
引用
收藏
页码:2883 / 2891
页数:9
相关论文
共 25 条
[1]  
[Anonymous], 1996, The molecular and cellular biology of wound repair
[2]   PRODUCTION OF A TISSUE-LIKE STRUCTURE BY CONTRACTION OF COLLAGEN LATTICES BY HUMAN-FIBROBLASTS OF DIFFERENT PROLIFERATIVE POTENTIAL INVITRO [J].
BELL, E ;
IVARSSON, B ;
MERRILL, C .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (03) :1274-1278
[3]  
Brown RA, 1998, J CELL PHYSIOL, V175, P323, DOI 10.1002/(SICI)1097-4652(199806)175:3<323::AID-JCP10>3.0.CO
[4]  
2-6
[5]  
Brown RA, 1996, J CELL PHYSIOL, V169, P439, DOI 10.1002/(SICI)1097-4652(199612)169:3<439::AID-JCP4>3.0.CO
[6]  
2-P
[7]  
Chapuis JF, 1996, EUR J DERMATOL, V6, P56
[8]   MEASUREMENT OF MECHANICAL FORCES GENERATED BY SKIN FIBROBLASTS EMBEDDED IN A 3-DIMENSIONAL COLLAGEN GEL [J].
DELVOYE, P ;
WILIQUET, P ;
LEVEQUE, JL ;
NUSGENS, BV ;
LAPIERE, CM .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1991, 97 (05) :898-902
[9]   ALPHA-SMOOTH MUSCLE ACTIN IS EXPRESSED IN A SUBPOPULATION OF CULTURED AND CLONED FIBROBLASTS AND IS MODULATED BY GAMMA-INTERFERON [J].
DESMOULIERE, A ;
RUBBIABRANDT, L ;
ABDIU, A ;
WALZ, T ;
MACIEIRACOELHO, A ;
GABBIANI, G .
EXPERIMENTAL CELL RESEARCH, 1992, 201 (01) :64-73
[10]   A CULTURE FORCE MONITOR FOR MEASUREMENT OF CONTRACTION FORCES GENERATED IN HUMAN DERMAL FIBROBLAST-CULTURES - EVIDENCE FOR CELL-MATRIX MECHANICAL SIGNALING [J].
EASTWOOD, M ;
MCGROUTHER, DA ;
BROWN, RA .
BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 1994, 1201 (02) :186-192