A common phosphorylation site for cyclic AMP-dependent protein kinase and protein kinase C in human placental 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase

被引：25

作者：

Okamura, N ^{[1
]}

Sakakibara, R ^{[1
]}

机构：

[1] Nagasaki Univ, Grad Sch Pharmaceut Sci, Dept Clin Pharmaceut, Nagasaki 8528131, Japan

来源：

BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY | 1998年 / 62卷 / 10期

关键词：

6-phosphofructo-2-kinase fructose-2,6-bis-phosphatase; human placenta; phosphorylation;

D O I：

10.1271/bbb.62.2039

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Human placental 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase (HP2K) was phosphorylated by incubation with [gamma-P-32]MgATP and cyclic AMP-dependent protein kinase (PKA) or protein I kinase C (PKC). Approximately 0.8 mol of phosphate per mol subunit of HP2K was incorporated by either PKA or PKC. However, with additional incubation with PEA following incubation with PKC or vice versa, no additional phosphate was incorporated into the HP2K. The phosphorylation sites for the two protein kinases were identified by peptide mapping and microsequencing following digestion of phosphorylated-HP2K with clostripain. Evidence is also suggested for a common phosphorylation site (Ser-460) for PKA and PKC.

引用

页码：2039 / 2042

页数：4

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