Diagnostic multiplex PCR assay for the identification of the Liverpool, Midlands 1 and Manchester CF epidemic strains of Pseudomonas aeruginosa

被引：28

作者：

Fothergill, Joanne L. ^{[1
]}

Upton, Anna L. ^{[1
]}

Pitt, Tyrone L. ^{[2
]}

Hart, C. Anthony ^{[1
]}

Winstanley, Craig ^{[1
]}

机构：

[1] Univ Liverpool, Div Med Microbiol, Liverpool L69 3GA, Merseyside, England

[2] Hlth Protect Agcy, Lab HealthCare Associated Infect, London NW9 5HT, England

来源：

JOURNAL OF CYSTIC FIBROSIS | 2008年 / 7卷 / 03期

关键词：

cystic fibrosis; Pseudomonas aeruginosa; epidemic strains; diagnostics;

D O I：

10.1016/j.jcf.2007.09.002

中图分类号：

R56 [呼吸系及胸部疾病];

学科分类号：

摘要：

Individual PCR amplification tests have been developed for three UK CF epidemic strains, the Liverpool epidemic strain (LES), Midlands 1 and the Manchester epidemic strain (MES). We report a simple diagnostic multiplex PCR test that can be used to screen for all three of these strains. To evaluate the test, we screened collections of LES, MES and Midlands I isolates, along with various CF and non-CF non-epidemic Pseudomonas aeruginosa strains. The test was 100% sensitive and 100% specific in the identification of these UK CF epidemic strains. (C) 2007 European Cystic Fibrosis Society. Published by Elsevier B.V. All rights reserved.

引用

页码：258 / 261

页数：4

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