A method for isolation of chloroplast DNA and mitochondrial DNA from sunflower

被引：86

作者：

Triboush, SO ^{[1
]}

Danilenko, NG ^{[1
]}

Davydenko, OG ^{[1
]}

机构：

[1] Natl Acad Sci, Inst Genet & Cytol, Dept Extranucl Hered, Minsk 220072, BELARUS

来源：

PLANT MOLECULAR BIOLOGY REPORTER | 1998年 / 16卷 / 02期

关键词：

chloroplast DNA; mitochondrial DNA; organelle isolation; sunflower;

D O I：

10.1023/A:1007487806583

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We present a method for isolation of chloroplast and mitochondrial DNA from sunflower seedlings. The protocol includes: organelle isolation, deoxyribonuclease treatment, lysis, deproteinisation and a final DNA purification with sodium dodecyl sulphate and potassium acetate. The oganelle DNA yield is 5-10 micrograms per gram of tissue and the DNA is fully restrictable. The technique is inexpensive and appropriate for the isolation of multiple samples of organelle DNA from a small amount of tissue.

引用

页码：183 / 189

页数：7

共 9 条

[1] PREPARATION OF CHLOROPLAST DNA FROM PEA PLASTIDS ISOLATED IN A MEDIUM OF HIGH IONIC-STRENGTH [J].

BOOKJANS, G ;

STUMMANN, BM ;

HENNINGSEN, KW .

ANALYTICAL BIOCHEMISTRY, 1984, 141 (01) :244-247

[2] MOLECULAR ANALYSIS OF THE MITOCHONDRIAL GENOME OF HELIANTHUS-ANNUUS IN RELATION TO CYTOPLASMIC MALE-STERILITY AND PHYLOGENY [J].

CROUZILLAT, D ;

LEROY, P ;

PERRAULT, A ;

LEDOIGT, G .

THEORETICAL AND APPLIED GENETICS, 1987, 74 (06) :773-780

[3]

Herrmann R.G., 1982, METHODS CHLOROPLAST, P259

[4]

KOHLER RH, 1991, MOL GEN GENET, V227, P369, DOI 10.1007/BF00273925

[5] DESOXYRIBONUCLEIC ACID PREPARATION BY FILTRATION ON AGAROSE GELS [J].

LOEB, JE ;

CHAUVEAU, J .

BIOCHIMICA ET BIOPHYSICA ACTA, 1969, 182 (01) :225-+

[6]

MACKENZIE SA, 1994, PLANT MOL BIOL MAN D, V3, P1

[7]

MALIGA P, 1995, METHODS PLANT MOL BI, P194

[8]

PALMER JD, 1986, METHOD ENZYMOL, V118, P167

[9] A RAPID INEXPENSIVE METHOD FOR THE ISOLATION OF RESTRICTABLE MITOCHONDRIAL-DNA FROM VARIOUS PLANT SOURCES [J].

WILSON, AJ ;

CHOUREY, PS .

PLANT CELL REPORTS, 1984, 3 (06) :237-239

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