Electron tomography of molecules and cells

被引：246

作者：

Baumeister, W ^{[1
]}

Grimm, R ^{[1
]}

Walz, J ^{[1
]}

机构：

[1] Max Planck Inst Biochem, Abt Mol Strukturbiol, D-82152 Martinsried, Germany

来源：

TRENDS IN CELL BIOLOGY | 1999年 / 9卷 / 02期

关键词：

D O I：

10.1016/S0962-8924(98)01423-8

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Electron tomography is the most winery applicable method for obtaining three-dimensional information by electron microscopy. It is, in fact, the only method suitable for investigating pleomorphic structures, such as many supramolecular assemblies, organelles and cells. With the recent development of automated low-dose data-acquisition schemes, it is now possible to study molecules and cells embedded in vitreous ice. This opens up new horizons for investigating the functional organization of cellular components with minimal perturbation of the cellular context.

引用

页码：81 / 85

页数：5

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