Expression of tropane alkaloids in the hairy root culture of Atropa acuminata substantiated by DART mass spectrometric technique

被引：22

作者：

Banerjee, Suchitra ^{[1
]}

Madhusudanan, K. P. ^{[2
]}

Chattopadhyay, Sunil K. ^{[1
]}

Rahman, Laiq Ur ^{[1
]}

Khanuja, Suman P. S. ^{[1
]}

机构：

[1] Cent Inst Med & Aromat Plants, Lucknow 226015, Uttar Pradesh, India

[2] Cent Drug Res Inst, Sophisticated Analyt Instrument Facil, Lucknow 226001, Uttar Pradesh, India

来源：

BIOMEDICAL CHROMATOGRAPHY | 2008年 / 22卷 / 08期

关键词：

Atropa acuminata; DART MS analysis; hairy root culture;

D O I：

10.1002/bmc.998

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Agrobacterium rhizogenes-mediated 'hairy root' cultures were established in Atropa acuminata. The chemical profiling of the hairy roots was carried out by a new mass spectrometric technique, direct analysis in real time (DART). The intact hairy roots were directly analyzed by holding them in the gap between the DART ion source and mass spectrometer. Two alkaloids, atropine and scopolamine, were characterized. The structural confirmation of the two alkaloids was made through their accurate molecular formula determinations. This is the first report of establishing hairy roots in A. acuminata as well as application of the DART technique for the chemical profiling of its hairy roots. Copyright (C) 2008 John Wiley & Sons, Ltd.

引用

页码：830 / 834

页数：5

共 25 条

[1]

[Anonymous], 1948, WEALTH INDIA DICT IN, V1, P135

[2]

Aoki T, 1997, PLANT CELL REP, V16, P282, DOI [10.1007/s002990050223, 10.1007/BF01088281]

[3] Expression of functional mammalian P450 2E1 in hairy root cultures [J].

Banerjee, S ;

Shang, TQ ;

Wilson, AM ;

Moore, AL ;

Strand, SE ;

Gordon, MP ;

Doty, SL .

BIOTECHNOLOGY AND BIOENGINEERING, 2002, 77 (04) :462-466

[4]

BANERJEE S, BIOMEICAL C IN PRESS

[5] Effects of the rol C gene on hairy root:: Induction development and tropane alkaloid production by Atropa belladonna [J].

Bonhomme, V ;

Laurain-Mattar, D ;

Fliniaux, MA .

JOURNAL OF NATURAL PRODUCTS, 2000, 63 (09) :1249-1252

[6] Bringing medicinal plants into cultivation: opportunities and challenges for biotechnology [J].

Canter, PH ;

Thomas, H ;

Ernst, E .

TRENDS IN BIOTECHNOLOGY, 2005, 23 (04) :180-185

[7] Versatile new ion source for the analysis of materials in open air under ambient conditions [J].

Cody, RB ;

Laramée, JA ;

Durst, HD .

ANALYTICAL CHEMISTRY, 2005, 77 (08) :2297-2302

[8] Integrated processes of extraction and liquid membrane isolation of atropine from Atropa belladonna roots [J].

Dimitrov, K ;

Metcheva, D ;

Boyadzhiev, L .

SEPARATION AND PURIFICATION TECHNOLOGY, 2005, 46 (1-2) :41-45

[9] Hairy root research:: recent scenario and exciting prospects -: Commentary [J].

Guillon, Stéphanie ;

Trémouillaux-Guiller, Jocelyne ;

Pati, Pratap Kumar ;

Rideau, Marc ;

Gantet, Pascal .

CURRENT OPINION IN PLANT BIOLOGY, 2006, 9 (03) :341-346

[10] PRODUCTION OF TROPANE ALKALOIDS IN GENETICALLY-ENGINEERED ROOT CULTURES [J].

HASHIMOTO, T ;

YUN, DJ ;

YAMADA, Y .

PHYTOCHEMISTRY, 1993, 32 (03) :713-718

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