Mutational analysis of the phosphate-binding loop of Rhizobium meliloti DctD, a σ54-dependent activator

被引：9

作者：

Gao, Y ^{[1
]}

Wang, YK ^{[1
]}

Hoover, TR ^{[1
]}

机构：

[1] Univ Georgia, Dept Microbiol, Athens, GA 30602 USA

来源：

JOURNAL OF BACTERIOLOGY | 1998年 / 180卷 / 10期

关键词：

D O I：

10.1128/JB.180.10.2792-2795.1998

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The phosphate-binding loop of sigma(54)-dependent activators is thought to participate in ATP binding and/or hydrolysis. Alanine substitutions at positions 3, 4, 6, 7, and 8 of this motif in Rhizobium meliloti DctD disrupted transcriptional activation and ATP hydrolysis. Interestingly, substitution of alanine at position 7 also affected DNA binding.

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页码：2792 / 2795

页数：4

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