Inhibitory effect of silibinin on tumour necrosis factor-alpha and hydrogen peroxide production by human monocytes

被引：21

作者：

Bannwart, C. F. ^{[1
]}

Peracoli, J. C. ^{[2
]}

Nakaira-Takahagi, E. ^{[1
]}

Peracoli, M. T. S. ^{[1
]}

机构：

[1] Sao Paulo State Univ, Dept Microbiol & Immunol, Biosci Inst, UNESP, BR-18618000 Botucatu, SP, Brazil

[2] Sao Paulo State Univ, Dept Gynecol & Obstet, Botucatu Med Sch, UNESP, BR-18618000 Botucatu, SP, Brazil

来源：

NATURAL PRODUCT RESEARCH | 2010年 / 24卷 / 18期

基金：

巴西圣保罗研究基金会;

关键词：

hydrogen peroxide; TNF-; flavonoid; silibinin; monocytes;

D O I：

10.1080/14786410903314492

中图分类号：

O69 [应用化学];

学科分类号：

070301 [无机化学];

摘要：

Silibinin is a chemically defined flavonoid and the main active component of silymarin, a polyphenolic complex from Silybum marianum, which has anti-inflammatory, hepatoprotective and anticarcinogenic properties. Monocytes obtained from healthy individuals were incubated with silibinin to evaluate cell viability, hydrogen peroxide (H2O2) release and tumour necrosis factor-alpha (TNF-) production by these cells. The duration of treatment and different silibinin concentrations had no significant effect on cell viability. Monocytes showed a dose-dependent inhibitory effect on H2O2 release by phorbol myristate acetate-stimulated monocytes in silibinin concentrations ranging from 6.25 to 50 mu g mL-1. Significant inhibition of TNF- production by lipopolysaccharide-stimulated monocytes was observed at concentrations of 12.5, 50 and 100 mu g mL-1 of silibinin. These results suggest that silibinin exerts antioxidant and anti-inflammatory properties on human monocytes through an inhibitory effect on H2O2 release and on TNF- production, respectively.

引用

页码：1747 / 1757

页数：11

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