Determination of aflatoxins in animal feeds by liquid chromatography/tandem mass spectrometry with isotope dilution

被引：24

作者：

Li, Wei

Herrman, Timothy J. ^{[2
]}

Dai, Susie Y. ^{[1
]}

机构：

[1] Texas A&M Univ, Off Texas State Chemist, Dept Vet Pathobiol, College Stn, TX 77843 USA

[2] Texas A&M Univ, Off Texas State Chemist, Dept Soil & Crop Sci, College Stn, TX 77843 USA

来源：

RAPID COMMUNICATIONS IN MASS SPECTROMETRY | 2011年 / 25卷 / 09期

关键词：

SUPERCRITICAL-FLUID EXTRACTION; LC-MS/MS; MYCOTOXIN ANALYSIS; INTERNAL STANDARD; PEANUT BUTTER; QUANTIFICATION; OCHRATOXIN; FOOD; MILK; M1;

D O I：

10.1002/rcm.4979

中图分类号：

Q5 [生物化学];

学科分类号：

070307 [化学生物学];

摘要：

The objective of the present study is to develop a simple, fast method for detection of aflatoxins in animal feeds. Simultaneous quantitation of four aflatoxins (AFB(1), AFB(2), AFG(1) and AFG(2)) in animal feeds was achieved in a single liquid chromatography/tandem mass spectrometry (LC/MS/MS) run. The solid-phase extraction cleanup step is eliminated with the stable isotope dilution method. Matrix effects were observed and overcome by isotope dilution. The method was tested in a variety of animal feed matrices and proved to be accurate and reliable. Method ruggedness tests resulted in recoveries of 78% to 122% with an intra-day assay precision of 2% to 15% and an inter-day assay precision of 3% to 17%. These results indicate that this method is suitable for quantitation of aflatoxins in animal feeds. Copyright (C) 2011 John Wiley & Sons, Ltd.

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页码：1222 / 1230

页数：9

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