Isolation and characterization of the exopolysaccharide produced by Streptococcus thermophilus SFi20

被引:39
作者
Navarini, L [1 ]
Abatangelo, A [1 ]
Bertocchi, C [1 ]
Conti, E [1 ]
Bosco, M [1 ]
Picotti, F [1 ]
机构
[1] POLY Tech SCARL, I-34012 Trieste, Italy
关键词
characterization; exopolysaccharide; isolation; Streptococcus thermophilus;
D O I
10.1016/S0141-8130(01)00118-0
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
This paper reports isolation, structural characterization and some physico-chemical properties in aqueous solution of the exopolysaccharide (EPS) produced by Streptococcus thermophilus strain SFi20. The yield of the purified EPS was found to be reproducible and close to the average value of 143 mg/l. The chemical structure, previously suggested, has been confirmed on the basis of NMR data. Viscometric, chiro-optical and rheological measurements have been carried out with the aim of characterizing the conformational state of the polysaccharide in aqueous solution. All the data reported indicate that the EPS does not undergo a cooperative conformational transition under the investigated experimental conditions. Furthermore, the viscosity data and the viscoelastic behaviour suggest that the polymer is rather flexible and adopts a random coil conformation in aqueous solution. (C) 2001 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:219 / 226
页数:8
相关论文
共 38 条
[1]   Solution properties of the exopolysaccharide produced by Pseudomonas marginalis strain HT041B [J].
Abatangelo, A ;
Gilli, R ;
Navarini, L ;
Rizzo, R ;
Osman, SF ;
Fett, WF .
JOURNAL OF CARBOHYDRATE CHEMISTRY, 1997, 16 (4-5) :583-598
[2]  
Bosco M, 2000, BIOPOLYMERS, V53, P272, DOI 10.1002/(SICI)1097-0282(200003)53:3<272::AID-BIP7>3.0.CO
[3]  
2-S
[4]   TEMPERATURE AND MOLECULAR-WEIGHT DEPENDENCE OF THE UNPERTURBED DIMENSIONS OF AQUEOUS PULLULAN [J].
BULIGA, GS ;
BRANT, DA .
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 1987, 9 (02) :71-76
[5]   COMPARATIVE FLEXIBILITY, EXTENSION, AND CONFORMATION OF SOME SIMPLE POLYSACCHARIDE CHAINS [J].
BURTON, BA ;
BRANT, DA .
BIOPOLYMERS, 1983, 22 (07) :1769-1792
[6]  
BUSH CA, 1981, ACS SYM SER, V150, P293
[7]   ISOLATION AND CHARACTERIZATION OF EXOCELLULAR POLYSACCHARIDE PRODUCED BY LACTOBACILLUS-BULGARICUS [J].
CERNING, J ;
BOUILLANNE, C ;
DESMAZEAUD, MJ ;
LANDON, M .
BIOTECHNOLOGY LETTERS, 1986, 8 (09) :625-628
[8]   EXOCELLULAR POLYSACCHARIDE PRODUCTION BY STREPTOCOCCUS-THERMOPHILUS [J].
CERNING, J ;
BOUILLANNE, C ;
DESMAZEAUD, MJ ;
LANDON, M .
BIOTECHNOLOGY LETTERS, 1988, 10 (04) :255-260
[9]  
CERNING J, 1990, FEMS MICROBIOL LETT, V87, P113, DOI 10.1111/j.1574-6968.1990.tb04883.x
[10]  
Cerning J, 1995, LAIT, V75, P463, DOI 10.1051/lait:19954-536