Mutations affecting DNA-binding activity of the MexR repressor of mexR-mexA-mexB-oprM operon expression

被引：28

作者：

Saito, K ^{[1
]}

Akama, H ^{[1
]}

Yoshihara, E ^{[1
]}

Nakae, T ^{[1
]}

机构：

[1] Tokai Univ, Sch Med, Dept Mol Life Sci, Isehara, Kanagawa 2591193, Japan

来源：

JOURNAL OF BACTERIOLOGY | 2003年 / 185卷 / 20期

关键词：

D O I：

10.1128/JB.185.20.6195-6198.2003

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We have isolated 25 MexR mutants that retained their dimerizing ability but were unable to bind mexOP DNA. Surprisingly, 20 mutations were located in the hydrophobic core region at alphaA, W1, alpha2, alpha3, and beta2, and only 3 were in positively charged residues. These results verified that DNA binding is mediated by distinct regions of MexR and showed the importance of the hydrophobic core region of the DNA-binding domain.

引用

页码：6195 / 6198

页数：4

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