Automated processing of forensic casework samples using robotic workstations equipped with nondisposable tips: Contamination prevention

被引：34

作者：

Fregeau, Chantal J. ^{[1
]}

Lett, C. Marc ^{[2
]}

Elliott, Jim ^{[2
]}

Yensen, Craig ^{[3
]}

Fourney, Ron M. ^{[1
]}

机构：

[1] Royal Canadian Mounted Police, Natl Serv & Res, Ottawa, ON K1G 3M8, Canada

[2] Royal Canadian Mounted Police, Forens Lab Serv, Ottawa, ON K1G 3M8, Canada

[3] Royal Canadian Mounted Police, Natl DNA Data Bank, Ottawa, ON K1G 3M8, Canada

来源：

JOURNAL OF FORENSIC SCIENCES | 2008年 / 53卷 / 03期

关键词：

forensic science; contamination; robotics; TECAN robots; casework; magnetic beads; DNA IQ (TM); fixed tips; D3S1358; HumvWA; HumFGA; D8S1179; D21S11; D18S51; D5S818; D13S317; D7S820;

D O I：

10.1111/j.1556-4029.2008.00697.x

中图分类号：

DF [法律]; D9 [法律]; R [医药、卫生];

学科分类号：

0301 ; 10 ;

摘要：

An automated process has been developed for the analysis of forensic casework samples using TECAN Genesis RSP 150/8 or Freedom EVO liquid handling workstations equipped exclusively with nondisposable tips. Robot tip cleaning routines have been incorporated strategically within the DNA extraction process as well as at the end of each session. Alternative options were examined for cleaning the tips and different strategies were employed to verify cross-contamination. A 2% sodium hypochlorite wash (1/5th dilution of the 10.8% commercial bleach stock) proved to be the best overall approach for preventing cross-contamination of samples processed using our automated protocol. The bleach wash steps do not adversely impact the short tandem repeat (STR) profiles developed from DNA extracted robotically and allow for major cost savings through the implementation of fixed tips. We have demonstrated that robotic workstations equipped with fixed pipette tips can be used with confidence with properly designed tip washing routines to process casework samples using an adapted magnetic bead extraction protocol.

引用

页码：632 / 651

页数：20

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